• Chandan Kumar Singh
• Md. Nizamuddin Ansari
• Nidhi Kumari
• Md.Naseem

Plants are the major source of food, fuel, fibre and fodder Besides these, they are also the sources of medicines, gene pool and aesthetic beauty. Increasing population, expanding urbanization, rapid industrialization and accelerated pace of developmental activities have resulted loss of valuable plant resources. A major concern of today is the rapid depletion of vasuable plant resources especially in the tropics (D’souza,1988; Tandon,1994; Masen, 1997; Barooh and Das, 2009). There is an urgent need for systematic cultivation and conservation of these economically important plants including crop plants.Tissue culture methods could help in the plant conservation. Conservation of plant diversity and germplasms of wild species of crop plants is, therefore, of utmost importance to ensure protection of a healthy environment and meeting basic human needs of food, health care, clothing and fuel (Fay,1992). The proper screening of wild relatives of crop plants including medicinal plants is the need of hour and their proper exploitation would need domestication and cultivation of these plants on large scale for scientific researches. Conservation and mass propagation of wild crops plants through tissue culture of wild crop plants including medicinal plants are being made to evaluate the morphogenic potentials for developing strategies for germplasm conservation as well as successful isolation and screening of secondary metabolites of pharmaceutical importance (Ahuja, 1994; Naseem and Jha, 1994, 1997; Chaturvedi and Sinha, 1979; Chaturvedi et al; 2004; Thind et al, 2008; Arya et al; 2009; Jadav, 2009; Kumar et al; 2010).

Germplasm, explants, tissue culture, shoot tip, callus, leaf primordia, Growth harmones, Auxin, Cytokinin.

"“Response of different growth harmones of leaf culture of solanum torvum”. ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.5, Issue 4, page no.1024-1042, April-2018, Available :http://www.jetir.org/papers/JETIR1804394.pdf

"“Response of different growth harmones of leaf culture of solanum torvum”. ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.5, Issue 4, page no. pp1024-1042, April-2018, Available at : http://www.jetir.org/papers/JETIR1804394.pdf