• T. Asha
• T. Selva Mohan

Microbial amylases have various industrial applications. A large number of these amylolytic enzymes are available commercially and have almost replaced chemical hydrolysis of starch in starch processing industry. The production of extracellular amylase by Citrobacter freundii was screened. The process parameters were optimized to enhance amylase production in submerged fermentation. The maximum production of amylase production was observed at 40°C (261 U/ml) by the strain Citrobacter freundii. The maximum production of amylase production was observed at pH 7 (219 U/ml). Among the carbon sources, cellulose enhanced enzyme production (58 U/ml). Among the tested nitrogen sources, peptone recorded the highest influence on amylase production (212 U/ml) by Citrobacter freundii.

Amylase, Citrobacter freundii, submerged fermentation.

"Screening and optimization of amylase from Citrobacter freundii isolated from the gut of Carassius auratus", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.5, Issue 10, page no.405-408, October-2018, Available :http://www.jetir.org/papers/JETIR1810670.pdf

"Screening and optimization of amylase from Citrobacter freundii isolated from the gut of Carassius auratus", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.5, Issue 10, page no. pp405-408, October-2018, Available at : http://www.jetir.org/papers/JETIR1810670.pdf