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Published in:

Volume 5 Issue 12
December-2018
eISSN: 2349-5162

Unique Identifier

JETIR1812964

Page Number

444-453

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Title

Characterization of the hemagglutinin in the hemolymph of the freshwater crab Travancoriana charu (Bahir and Yeo,2007)

ISSN

2349-5162

Cite This Article

"Characterization of the hemagglutinin in the hemolymph of the freshwater crab Travancoriana charu (Bahir and Yeo,2007)", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.5, Issue 12, page no.444-453, December-2018, Available :http://www.jetir.org/papers/JETIR1812964.pdf

Abstract

The hemolymph agglutinin of the freshwater crab Travancoriana charu recognized with high avidity the simple sugar galactose and the amino sugar N-acetyl glucosamine. The sialoglycoprotein BSM was the strongest glycoprotein inhibitor. Hemagglutination activity (HA) was characterized using several kinds of mammalian erythrocytes and the highest hemagglutination titer was observed against dog erythrocytes containing NeuGc / NeuAc. Maximum hemagglutination of the calcium dependent hemagglutinin was observed at pH 7.5 and temperature 30ᵒC. The agglutinin was sensitive to di- / tetra sodium EDTA and trisodium citrate. Protease treatment enhanced the hemagglutinability. However, treatment with sialidase and denaturing agents caused a decline in the HA titer. Further, cross adsorption studies suggested the presence of a single agglutinin in the hemolymph.

Key Words

Hemagglutination, lectin, Travancoriana charu, sialoglycoprotein, agglutinin

Cite This Article

"Characterization of the hemagglutinin in the hemolymph of the freshwater crab Travancoriana charu (Bahir and Yeo,2007)", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.5, Issue 12, page no. pp444-453, December-2018, Available at : http://www.jetir.org/papers/JETIR1812964.pdf

Publication Details

Published Paper ID: JETIR1812964
Registration ID: 194241
Published In: Volume 5 | Issue 12 | Year December-2018
DOI (Digital Object Identifier):
Page No: 444-453
ISSN Number: 2349-5162

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Cite This Article

"Characterization of the hemagglutinin in the hemolymph of the freshwater crab Travancoriana charu (Bahir and Yeo,2007)", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.5, Issue 12, page no. pp444-453, December-2018, Available at : http://www.jetir.org/papers/JETIR1812964.pdf




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