• Dr. Jyoti mehta
• Mr. Mohd Akhlak

AIM: Microbial populations in indoor environments, where we live and eat, are important for public health. So the purpose of this study is to isolate and identify the bacterial contamination from common household. METHODS: Five samples of bacteria were collected from pillow cover, bed sheets, bathroom floor, laptop, and wash basin. Each sample was cultured on the nutrient agar plates and cultural characterization was done on the basis of colony morphology and gram staining. Each sample was further characterized on the basis of standard biochemical tests and its antibiotic sensitivity was tested against antibiotics (Norfloxacin, Azithromycin, Sulphamethoxazole, Cefixime, Tetracycline, Penicillin, Ciprofloxacin, Oxacillin and Cefuroxime) using disc diffusion method. RESULTS: Gram-negative and gram positive bacilli which appeared rod-shaped pinkish and purple colored colonies appeared under the microscope. Five isolates were characterized on the basis of biochemical tests that showed Gram-positive (S.aureus and S.pneumoniae) and Gram-negative (Shigella, E.coli and K.pneumoniae) bacteria in the isolated samples from household surfaces. The results of microbiological tests of household goods can be used to emphasize the importance of the sanitary conditions in the house.

Antibiotics, disc diffusion method, gram staining

"ISOLATION AND IDENTIFICATION OF BACTERIAL CONTAMINATION FROM COMMONLY USED HOUSEHOLD SURFACES", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.5, Issue 12, page no.697-705, December-2018, Available :http://www.jetir.org/papers/JETIR1812A95.pdf

"ISOLATION AND IDENTIFICATION OF BACTERIAL CONTAMINATION FROM COMMONLY USED HOUSEHOLD SURFACES", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.5, Issue 12, page no. pp697-705, December-2018, Available at : http://www.jetir.org/papers/JETIR1812A95.pdf