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Published in:

Volume 5 Issue 12
December-2018
eISSN: 2349-5162

Unique Identifier

JETIR1812B77

Page Number

611-620

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Title

Analytical Approach for Development and Validation of Stability Indicating RP-HPLC Method for Simultaneous Estimation of Saxagliptin and Dapagliflozin in Pharmaceutical Dosage Form

ISSN

2349-5162

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"Analytical Approach for Development and Validation of Stability Indicating RP-HPLC Method for Simultaneous Estimation of Saxagliptin and Dapagliflozin in Pharmaceutical Dosage Form", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.5, Issue 12, page no.611-620, December-2018, Available :http://www.jetir.org/papers/JETIR1812B77.pdf

Abstract

A simple, rapid, economical, precise and accurate stability indicting HPLC method for simultaneous estimation of Dapagliflozin and Saxagliptin in their combined dosage form has been developed. A stability indicting high performance liquid chromatographic method was developed for the simultaneous estimation of Dapagliflozin and Saxagliptin in their combined dosage form has been developed. The separation was achieved by LC- 20 AT C18 (250mm x 4.6 mm x 2.6 µm) column and Buffer (pH 6.0): Acetonitrile (70:30) at a flow rate of 1 ml/min. Detection was carried out at 275 nm. Retention time 3.593 min and 5.263 for Dapagliflozin and Saxagliptin respectively. The method has been validated for linearity, accuracy and precision. Linearity observed for Dapagliflozin 10-30 μg/ml and for Saxagliptin 5-15 μg/ml. Developed method was found to be accurate, precise and rapid for simultaneous estimation of Dapagliflozin and Saxagliptin in their combined dosage form. The proposed method was successfully applied for the simultaneous estimation of both the drugs in commercial Combined dosage form.

Key Words

Dapagliflozin, Saxagliptin, RP-HPLC Method Development, Validation, Forced Degradation

Cite This Article

"Analytical Approach for Development and Validation of Stability Indicating RP-HPLC Method for Simultaneous Estimation of Saxagliptin and Dapagliflozin in Pharmaceutical Dosage Form", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.5, Issue 12, page no. pp611-620, December-2018, Available at : http://www.jetir.org/papers/JETIR1812B77.pdf

Publication Details

Published Paper ID: JETIR1812B77
Registration ID: 194465
Published In: Volume 5 | Issue 12 | Year December-2018
DOI (Digital Object Identifier):
Page No: 611-620
ISSN Number: 2349-5162

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Cite This Article

"Analytical Approach for Development and Validation of Stability Indicating RP-HPLC Method for Simultaneous Estimation of Saxagliptin and Dapagliflozin in Pharmaceutical Dosage Form", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.5, Issue 12, page no. pp611-620, December-2018, Available at : http://www.jetir.org/papers/JETIR1812B77.pdf




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