• Patel Seju D.
• Dr. Pragnesh Patani

A simple, rapid, economical, precise and accurate stability indicting HPLC method for simultaneous estimation of Dapagliflozin and Saxagliptin in their combined dosage form has been developed. A stability indicting high performance liquid chromatographic method was developed for the simultaneous estimation of Dapagliflozin and Saxagliptin in their combined dosage form has been developed. The separation was achieved by LC- 20 AT C18 (250mm x 4.6 mm x 2.6 µm) column and Buffer (pH 6.0): Acetonitrile (70:30) at a flow rate of 1 ml/min. Detection was carried out at 275 nm. Retention time 3.593 min and 5.263 for Dapagliflozin and Saxagliptin respectively. The method has been validated for linearity, accuracy and precision. Linearity observed for Dapagliflozin 10-30 μg/ml and for Saxagliptin 5-15 μg/ml. Developed method was found to be accurate, precise and rapid for simultaneous estimation of Dapagliflozin and Saxagliptin in their combined dosage form. The proposed method was successfully applied for the simultaneous estimation of both the drugs in commercial Combined dosage form.

Dapagliflozin, Saxagliptin, RP-HPLC Method Development, Validation, Forced Degradation

"Analytical Approach for Development and Validation of Stability Indicating RP-HPLC Method for Simultaneous Estimation of Saxagliptin and Dapagliflozin in Pharmaceutical Dosage Form", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.5, Issue 12, page no.611-620, December-2018, Available :http://www.jetir.org/papers/JETIR1812B77.pdf

"Analytical Approach for Development and Validation of Stability Indicating RP-HPLC Method for Simultaneous Estimation of Saxagliptin and Dapagliflozin in Pharmaceutical Dosage Form", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.5, Issue 12, page no. pp611-620, December-2018, Available at : http://www.jetir.org/papers/JETIR1812B77.pdf