• Dr. Sandip Nanusingh Chavan

The present study was listening carefully on evaluation of antimicrobial activity of Ocimum sanctum leaf extract in normal tap water and local river water. The antimicrobial result was calculated with different concentration (100 to 600 mg l-1) of tulsi leaf extract in tap water. In this case it contains 600 mg l-1 concentration of plant extract treated with water and show effective antimicrobial activity at 15 to 16 hrs than after that the other concentration extracts. It also contains 500 mg, l-1 of extract treated with water showed 95 to 98% antibacterial activity in 14 to 16 hrs. Tulsi is a Sanskrit word which means “matchless one”. Several medicinal properties have been credited to the Tulsi plant. In ayurveda, tulsi used as anti asthmatic drugs. It is also used in treatment of fever, bronchitis, arthritis, convulsion etc. Scientific explorations of traditional belief of medicinal properties of Tulsi have got momentum mostly after the middle of the 20th century. Ocimum sanctum (Tulsi or holy basil) has a very special place in the Hindu culture. The minimum bacterial concentration (MBC) was observed in 500 and 600 mg l-1 extract concentration. The concentration of the bacterial cells reserved gradually for an hour was studied by spread plate method.

Minimum Bactericidal Concentration, Microbial growth. Ocimum sanctum, Antimicrobial effect,

"Ocimum sanctum removes impurities from water and plays disinfectant role", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.7, Issue 2, page no.490-494, February-2020, Available :http://www.jetir.org/papers/JETIR2002277.pdf

"Ocimum sanctum removes impurities from water and plays disinfectant role", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.7, Issue 2, page no. pp490-494, February-2020, Available at : http://www.jetir.org/papers/JETIR2002277.pdf