• Dr. S.V.AHIRE

Gloriosa superba L. also known as Malabar glory lily is a commercially important medicinal plant which has diverse medicinal applications and eventually due toover-exploitation this plant is facing local extinction. Plant tissue culture technologyincluding micro propagation has much potential in facilitating experimental studies to gain a better understanding of thebiology of endangered plants. In the present research work, the phytochemical composition of Callus, leaves and tuber roots was investigated using different phytochemical methods.The work is divided in two parts: in vitro propagation and phytochemical analysis. The explants used for in vitropropagation are leaf and fresh tuber roots. Maximum callus was obtained in M.S medium supplemented with BAP (1.5-3 mg/l) in combination with NAA (0.2-0.5 mg/l) Organogenesis responses better in M.S. medium supplemented with N.A.A. (0.5 mg/l) and BAP (1.5 mg/l). Obtained callus, fresh tuber roots and leaves are further used for phytochemical analysis which includes the qualitative and quantitative analysis of reducing sugar,alkaloids, proteins, and nucleic acid (DNA and RNA) .

Gloriosa superba L., Phytochemical, InVitro propagation

"A comparative phytochemical composition of Gloriosa superba L. callus tissues and it’s natural leaf and fresh tuber roots extracts", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.7, Issue 3, page no.1313-1316, March-2020, Available :http://www.jetir.org/papers/JETIR2003193.pdf

"A comparative phytochemical composition of Gloriosa superba L. callus tissues and it’s natural leaf and fresh tuber roots extracts", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.7, Issue 3, page no. pp1313-1316, March-2020, Available at : http://www.jetir.org/papers/JETIR2003193.pdf