JETIREXPLORE- Search Thousands of research papers



Published in:

Volume 7 Issue 5
May-2020
eISSN: 2349-5162

Unique Identifier

JETIR2005073

Page Number

481-487

Share This Article


Title

“ISOLATION OF “ISOLATION OF ANTIBIOTIC PRODUCING ACTINOMYCETES FROM SOIL”

ISSN

2349-5162

Cite This Article

"“ISOLATION OF “ISOLATION OF ANTIBIOTIC PRODUCING ACTINOMYCETES FROM SOIL”", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.7, Issue 5, page no.481-487, May-2020, Available :http://www.jetir.org/papers/JETIR2005073.pdf

Abstract

The present study deals with the isolation, production, purification and optimization of antibiotics from soil. The soil samples were collected from various places. Actinomycetes strains were isolated in specific medium using starch casein agar medium. The actinomycetes were screened with regard to potential against gram positive and gram negative bacteria. The purified actinomycetes strains were performed in biochemical tests. Gram staining, sugars fermentation, HS production, urease production, MR-VP test, indole production, citrate 2 utilization. Fermentation was carried out with starch casein broth and identified strain of actinomyces as inoculums. Antimicrobial activity of antibiotics was detected on Mueller Hinton Agar Media against E.coli. The purification of antibiotics was done with the help of activated charcoal. During the experiment a modified fermentation medium was formulated and activity of antibiotic produced in this medium was detected against E.coli. The isolated bacteria such as actinomycetesobtained from the soil.

Key Words

“ISOLATION OF ANTIBIOTIC PRODUCING ACTINOMYCETES FROM SOIL”

Cite This Article

"“ISOLATION OF “ISOLATION OF ANTIBIOTIC PRODUCING ACTINOMYCETES FROM SOIL”", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.7, Issue 5, page no. pp481-487, May-2020, Available at : http://www.jetir.org/papers/JETIR2005073.pdf

Publication Details

Published Paper ID: JETIR2005073
Registration ID: 231477
Published In: Volume 7 | Issue 5 | Year May-2020
DOI (Digital Object Identifier):
Page No: 481-487
ISSN Number: 2349-5162

Download Paper

Preview Article

Download Paper




Cite This Article

"“ISOLATION OF “ISOLATION OF ANTIBIOTIC PRODUCING ACTINOMYCETES FROM SOIL”", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.7, Issue 5, page no. pp481-487, May-2020, Available at : http://www.jetir.org/papers/JETIR2005073.pdf




Preview This Article


Downlaod

Click here for Article Preview

Download PDF

Downloads

00042

Print This Page