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Published in:

Volume 7 Issue 6
June-2020
eISSN: 2349-5162

Unique Identifier

JETIR2006164

Page Number

1154-1159

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Title

Isolation of nitrifying bacteria from oil contaminated soil to study their biodegradability and enhancing crude oil degradation with the use of selected bulking agents

ISSN

2349-5162

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"Isolation of nitrifying bacteria from oil contaminated soil to study their biodegradability and enhancing crude oil degradation with the use of selected bulking agents", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.7, Issue 6, page no.1154-1159, June-2020, Available :http://www.jetir.org/papers/JETIR2006164.pdf

Abstract

Oil exploration activities have consequential environmental impact on air, water and land. The present study deals with crude oil degrading potential of nitrifying bacteria isolated from oil contaminated soil near Valsad District. Total 21 isolates were obtained using Winogradsky medium and further 14 oil degrading isolates were obtained using Mineral basal medium with crude oil as carbon and energy source. 5 isolates OCSAz1, OCS1, GDSAz1, OCS-C and OCS-D was used to study their oil degrading activity. Inoculum size of OCSAz1 was 1.0 × 107cfu/ml and OCS-1 was 1.25 × 106 cfu/ml. Temperature, pH, optical density, total viable cells by SPC and residual oil were measured gravimetrically for these isolates. Nitrifying bacteria successfully utilize crude oil as carbon and energy source and also able to utilize engine oil, toluene, phenol and kerosene. From these isolates, OCSAz1 showed highest activity with 45% degradation rate with total cell count of 4.68 × 108cfu/ml after 15 days of degradation. Followed by this, OCS1 showed 40% degradation rate with total viable cells of 3.35 × 108cfu/ml. OCSAz1 and OCS-1 gave their activity at temperature of 27 C and pH value of 4.0 and 5.5, respectively. Result of BATH test showed adherence ability of OCSAz1 with 45 % and OCS1 with 20 %. The degradation rate was further enhanced by using bulking agents such as Rice husk, Sugarcane bagasse, vermicompost and NPK. Rice husk treated medium gave best result with 67% of degradation with total viable cells of 2.1×108 cfu/ml within 96 hours followed by sugarcane bagasse gave 60% degradation with total viable cells of 1.2×108 cfu/ml. The high crude oil utilization of isolate implies that nitrifying bacteria isolated from contaminated ecosystem are excellent crude oil degraders and can be used for bioremediation purpose.

Key Words

Biodegradation, Nitrifying bacteria, Crude oil, Winogradsky, Bioremediation.

Cite This Article

"Isolation of nitrifying bacteria from oil contaminated soil to study their biodegradability and enhancing crude oil degradation with the use of selected bulking agents", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.7, Issue 6, page no. pp1154-1159, June-2020, Available at : http://www.jetir.org/papers/JETIR2006164.pdf

Publication Details

Published Paper ID: JETIR2006164
Registration ID: 234142
Published In: Volume 7 | Issue 6 | Year June-2020
DOI (Digital Object Identifier): http://doi.one/10.1729/Journal.23776
Page No: 1154-1159
ISSN Number: 2349-5162

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Cite This Article

"Isolation of nitrifying bacteria from oil contaminated soil to study their biodegradability and enhancing crude oil degradation with the use of selected bulking agents", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.7, Issue 6, page no. pp1154-1159, June-2020, Available at : http://www.jetir.org/papers/JETIR2006164.pdf




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