• Sayali Satyendra Pawar
• Arunadevi S Birajdar
• Amol A Joshi
• Sushma G Panchal
• Vinayak A Shinde

Reverse phase high performance liquid chromatographic was developed for the estimation of Clobetasol Propionate in bulk & Formulation and to validate the developed methods according to ICH Q2 (R1) guidelines. Analytical method development was started with preliminary studies of the Clobetasol Propionate according to USP 2010. Being freely soluble in Acetonitrile, stock solutions of the drugs were prepared in acetonitrile (100%). The RP-HPLC method for estimation of Clobetasol Propionate dosage form was developed. The quantification was carried out by using Phenomenex Luna C18 column (150 mm × 4.6 mm, 5 m) as stationary phase and Acetonitrile: Water (51:49) as mobile phase. Mobile phase was maintained at a flow rate of 0.8 ml/min. The UV detector was operated at 242 nm at 30oC and Clobetasol propionate eluted at RT 15.7 min.

Clobetasol propionate,hplc,validation

"RP-HPLC Method Development and Validation of Clobetasol Propionate In Bulk and Gel Formulation", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.7, Issue 11, page no.867-876, November-2020, Available :http://www.jetir.org/papers/JETIR2011255.pdf

"RP-HPLC Method Development and Validation of Clobetasol Propionate In Bulk and Gel Formulation", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.7, Issue 11, page no. pp867-876, November-2020, Available at : http://www.jetir.org/papers/JETIR2011255.pdf