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Published in:

Volume 8 Issue 3
March-2021
eISSN: 2349-5162

Unique Identifier

JETIR2103010

Page Number

65-72

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Title

Serological and Molecular Diagnosis of Emerging Dengue Infection at a Tertiary Care Hospital in Kanpur, India

ISSN

2349-5162

Cite This Article

"Serological and Molecular Diagnosis of Emerging Dengue Infection at a Tertiary Care Hospital in Kanpur, India", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.8, Issue 3, page no.65-72, March-2021, Available :http://www.jetir.org/papers/JETIR2103010.pdf

Abstract

Dengue is a worldly common mosquito-borne disease. Dengue fever presents clinical characteristics similar to other febrile illness. Thus laboratory diagnosis is important for adequate management of the disease. Material and Methods: Clinical data and blood samples were collected from suspected patients with dengue who attended Rama Hospital. Serologic methods; ELISA test were performed to detect NS1, IgM and IgG and real-time PCR were performed in recent acute dengue illness serum samples for molecular typing. Result: Of 400 samples, 284 were diagnosed positive. 96(24%) were indicate sero-positivity with NS1 antigen by ELISA. IgM and IgG were diagnosed in 111(27.75%) and 150(37.5%) respectively. The major clinical presentation was a febrile illness, myalagia, low blood pressure, low hemoglobin count, thrombocytopenia and elevated liver enzymes. Majority of Dengue positive cases were reported in post monsoon season. In recent acute dengue virus infection cases, 06 and 04 cases were positive for DEN-2 and 04 DEN-3 respectively. The median of Ct value of DEN 2 and DEN 3 were 20.4 and 22.7 respectively. No DEN-1 and DEN-4 were found in this study. Conclusion: According to our study only DEN 2 and DEN in Kanpur during the study period. But the epidemiology of dengue virus and its prevalent serotypes are changing. Therefore continuous detection of prevalence of different serotypes is necessary for monitoring the severity of different serotypes and its management.

Key Words

Dengue fever, DEN-1, DEN-2, RT-PCR

Cite This Article

"Serological and Molecular Diagnosis of Emerging Dengue Infection at a Tertiary Care Hospital in Kanpur, India", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.8, Issue 3, page no. pp65-72, March-2021, Available at : http://www.jetir.org/papers/JETIR2103010.pdf

Publication Details

Published Paper ID: JETIR2103010
Registration ID: 306317
Published In: Volume 8 | Issue 3 | Year March-2021
DOI (Digital Object Identifier):
Page No: 65-72
ISSN Number: 2349-5162

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Cite This Article

"Serological and Molecular Diagnosis of Emerging Dengue Infection at a Tertiary Care Hospital in Kanpur, India", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.8, Issue 3, page no. pp65-72, March-2021, Available at : http://www.jetir.org/papers/JETIR2103010.pdf




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