• Sonali Patil
• Geetha Unnikrishnan

Thermophiles have developed a number of mechanisms to resist the toxic effects of heavy metals. The efficiency of bioremediation by thermophiles is dependent on the presence of biochemical factors present in the cell; like protein/ enzyme, EPS (Extra polymeric substances) or biogenic volatile substances. The first mechanisms by which bioremediation of toxic substances take place in microorganisms is with the help of protein/ enzymes. In the present study, heavy metal tolerating, new isolate of Pseudomonas fluorescens SONALIZANKAR was identified and the strain was deposited in NCBI database with accession number KM527212. The molecular mechanisms underlying the bioremediation phenomenon and the differential expression of proteins in response to the metal stress was also carried out. The band pattern in SDS-PAGE showed the difference in pattern of protein expression in presence and absence of heavy metals. Some proteins were over expressed in presence of heavy metals and some new bands were formed that indicates important role of proteins/enzymes during stress conditions.

Thermophiles, protein profiling, Bioremediation, heavy Metals, NCBI

"Protein profiling of heavy metal tolerating new strain of Pseudomonas isolated from hot water spring", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 5, page no.193-201, May-2019, Available :http://www.jetir.org/papers/JETIRCB06030.pdf

"Protein profiling of heavy metal tolerating new strain of Pseudomonas isolated from hot water spring", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 5, page no. pp193-201, May-2019, Available at : http://www.jetir.org/papers/JETIRCB06030.pdf