• Dr. Shanta P. Janyani
• Dr. A. K. Singh

Histopathological technique is a very old technique of making sections of the tissues or organs, so that the structure of cells can be visualized under the microscope. Histopathological alterations in the structure of cells indicate the physiological state of the cell and hence help in the diagnosis of the disease. With the advent of technology, camera microscope gives a clear image of the cells indicating their physiological state. Camera microscope enables us to capture live images, seen on computer screen & hence helps the investigator to analyse the data based on histopathological differences instantly. A 40X magnification of camera microscope revealed huge differences in the histological structure of liver and alimentary canal of Channa punctatus in response to clove oil. Channa punctatus is an air-breathing fresh water fish, which can be acclimated in the laboratory conditions with ease. Clove oil is an anesthetic agent used since immemorial times in medicine (dentistry). Clove oil seduces the fish and decreases its mobility to help in handling procedures during transportation. On exposure to clove oil for 32 days, it was observed that the liver cells membrane got disintegrated gradually with increasing duration of clove oil. The alimentary canal also showed atrophy of the cells. The gastric glands chickened and lost their ability to secrete. The inner lining of the stomach and intestine became congruent indicating it’s degradation & hence ability to lose function. Hence, it was concluded that clove oil cannot be used as an anesthetic agent for fish during transportation.

Channa punctatus, clove oil, liver, intestine, histopathological alterations, degradation.

"Toxicity analysis of histopathological alterations in liver and alimentary canal of Channa punctatus in response to clove oil as an anaesthetic agent", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 5, page no.213-217, May-2019, Available :http://www.jetir.org/papers/JETIRCB06033.pdf

"Toxicity analysis of histopathological alterations in liver and alimentary canal of Channa punctatus in response to clove oil as an anaesthetic agent", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 5, page no. pp213-217, May-2019, Available at : http://www.jetir.org/papers/JETIRCB06033.pdf