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Published in:

Volume 7 Issue 3
March-2020
eISSN: 2349-5162

Unique Identifier

JETIRDN06021

Page Number

106-113

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Title

RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR THE ESTIMATION OF SAXAGLIPTIN & DAPAGLIFLOZIN IN PHARMACEUTICAL DOSAGE FORM

ISSN

2349-5162

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"RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR THE ESTIMATION OF SAXAGLIPTIN & DAPAGLIFLOZIN IN PHARMACEUTICAL DOSAGE FORM", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.7, Issue 3, page no.106-113, March-2020, Available :http://www.jetir.org/papers/JETIRDN06021.pdf

Abstract

A new, simple and accurate, precise RP-HPLC method was developed for simultaneous determination of Dapagliflozin and Saxagliptinin bulk and in pharmaceutical dosage form. The separation of Dapagliflozin and Saxagliptin was achieved within 8 minutes on Symmetry C18 150mm X 4.6mm and 5µm Particle Size, Make Waters column using Methanol: Water (75:25v/v) as the mobile phase. Detection was carried out using wavelength at 270nm. Retention time of Dapagliflozin and Saxagliptinwas found to be 2.029 and 3.290min, respectively. The validation of the developed method was performed in terms of accuracy, precision, linearity, limit of detection, limit of quantification as mentioned in International Conference on Harmonization (ICH) guidelines. The method showed adequate sensitivity concerning linearity, accuracy and precision over the range 50-150μg/ml and 25-75μg/ml for Dapagliflozin and Saxagliptin, respectively. The percentage recoveries obtained for Dapagliflozin and Saxagliptin were found to be in range of 98.00 – 102.00 %. The proposed method is hence suitable for use in quality-control laboratories for quantitative analysis of both the drugs bulk and in combination, since it is simple and fast with good accuracy and precision.

Key Words

Dapagliflozin and Saxagliptin, RP-HPLC, Accuracy, Precision.

Cite This Article

"RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR THE ESTIMATION OF SAXAGLIPTIN & DAPAGLIFLOZIN IN PHARMACEUTICAL DOSAGE FORM", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.7, Issue 3, page no. pp106-113, March-2020, Available at : http://www.jetir.org/papers/JETIRDN06021.pdf

Publication Details

Published Paper ID: JETIRDN06021
Registration ID: 229522
Published In: Volume 7 | Issue 3 | Year March-2020
DOI (Digital Object Identifier):
Page No: 106-113
ISSN Number: 2349-5162

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Cite This Article

"RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR THE ESTIMATION OF SAXAGLIPTIN & DAPAGLIFLOZIN IN PHARMACEUTICAL DOSAGE FORM", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.7, Issue 3, page no. pp106-113, March-2020, Available at : http://www.jetir.org/papers/JETIRDN06021.pdf




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