• G.M.Kadam
• A.L.Puyad
• T.M. Kalyankar

A new, simple and accurate, precise RP-HPLC method was developed for simultaneous determination of Dapagliflozin and Saxagliptinin bulk and in pharmaceutical dosage form. The separation of Dapagliflozin and Saxagliptin was achieved within 8 minutes on Symmetry C18 150mm X 4.6mm and 5µm Particle Size, Make Waters column using Methanol: Water (75:25v/v) as the mobile phase. Detection was carried out using wavelength at 270nm. Retention time of Dapagliflozin and Saxagliptinwas found to be 2.029 and 3.290min, respectively. The validation of the developed method was performed in terms of accuracy, precision, linearity, limit of detection, limit of quantification as mentioned in International Conference on Harmonization (ICH) guidelines. The method showed adequate sensitivity concerning linearity, accuracy and precision over the range 50-150μg/ml and 25-75μg/ml for Dapagliflozin and Saxagliptin, respectively. The percentage recoveries obtained for Dapagliflozin and Saxagliptin were found to be in range of 98.00 – 102.00 %. The proposed method is hence suitable for use in quality-control laboratories for quantitative analysis of both the drugs bulk and in combination, since it is simple and fast with good accuracy and precision.

Dapagliflozin and Saxagliptin, RP-HPLC, Accuracy, Precision.

"RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR THE ESTIMATION OF SAXAGLIPTIN & DAPAGLIFLOZIN IN PHARMACEUTICAL DOSAGE FORM", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.7, Issue 3, page no.106-113, March-2020, Available :http://www.jetir.org/papers/JETIRDN06021.pdf

"RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR THE ESTIMATION OF SAXAGLIPTIN & DAPAGLIFLOZIN IN PHARMACEUTICAL DOSAGE FORM", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.7, Issue 3, page no. pp106-113, March-2020, Available at : http://www.jetir.org/papers/JETIRDN06021.pdf