• Haruna Karamba
• Anand Mohan
• Madhuri Girdhar

The current research aimed at analyzing quantification and optimization of polyhydroxyalkanoate (PHA) in bioplastic production for controlling environmental pollutions course by petroleum derived plastics. The water and soil sample were used in order to isolate the PHB producing organisms, the medium used is modified mineral salt broth for the culturing and production of PHB produce organisms. Some amount of salt (NaCl) in different concentration is added to the medium during preparation since PHB producing organisms grow only in the present of salt. Bacterial growth and bar chart graphs were also plotted. The isolated organisms from these samples are gram positive and gram negative bacilli. The quantification analysis of these bacterial strains at different carbon sources showed that the highest PHB yield percentage is occurred in the medium containing lactose as source of carbon. The best temperature for the growing of these bacterial strains is 37oC. PHB recovery and extraction of these bacterial strains was carried out using chloroform extraction method. Fourier transform infrared (FTIR) and thin layer chromatography (TLC) analysis of the extracted PHB was also performed. Biochemical analysis was done for the tentative identification of these bacterial strains. Bio-plastics are bio-based, biodegradable plastics with nearly similar properties to synthetic plastics. So bio-plastics are used in assisting the world to reduce the escalating harms of litter mostly in the rivers, sand and seas.

"Strategical Approaches for Quantification and Optimization for Polyhydroxybutyrate Production from Bacterial Strains of Salty Water Body Origin from South west of Lagos State, Nigeria", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.5, Issue 12, page no.1136-1149, December 2018, Available :http://www.jetir.org/papers/JETIRDZ06147.pdf

"Strategical Approaches for Quantification and Optimization for Polyhydroxybutyrate Production from Bacterial Strains of Salty Water Body Origin from South west of Lagos State, Nigeria", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.5, Issue 12, page no. pp1136-1149, December 2018, Available at : http://www.jetir.org/papers/JETIRDZ06147.pdf