• Hardik S. Churi
• Prof. Shailaja Palan

Marine bacteria though rich in pigments largely remain unexplored for its bioactive components. Synthetic pigments used in various industries pose various harmful effects, to overcome these problems it is important to explore varied natural sources for natural pigments. Pigmented bacterial isolates were screened for sediment sample of the coastal region of kelwe beach,palghar. 20 different bacterial isolates were obtained on st.zobells agar. From these 20 bacterial isolates, 2 visually pigmented bacteria orange and yellow pigment producing bacteria were further studied. The MIC of both microbial isolates were found to be at 8% NaCl and hence was moderate halotolerant in nature. Optimum growth of both pigment producing bacteria was found to be at pH 9. Pigment was extracted from bacteria using different solvents such as methanol, ethanol, acetone and chloroform, the maximum absorbance of the pigment was found to be 430nm and methanol was found to be the best solvent for maximum pigment extraction for both the isolates. The identification of pigment was done by chromatography technique and the Rf value was 0.48 and 0.58 for orange and yellow pigment, probably may contain zeaxanthin and lutein respectively. Antioxidant activity of pigment was checked by DPPH method, the percentage of scavenging activity of orange and yellow pigment was found to be 18.841% and 33.334% respectively. Antimicrobial activity of the extracted pigment was studied against E.coli, S.aureus and S.typhi by agar cup method and both pigment show maximum antimicrobial activity against only S.aureus

Marine pigments, thin layer chromatography, Antioxidant, DPPH assay, Antimicrobial activity.

"IDENTIFICATION AND CHARACTERIZATION OF BIOACTIVE PIGMENTS FROM MARINE BACTERIA ISOLATED FROM COASTAL REGION OF KELWE-MAHIM, PALGHAR", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.7, Issue 11, page no.24-31, November-2020, Available :http://www.jetir.org/papers/JETIREI06004.pdf

"IDENTIFICATION AND CHARACTERIZATION OF BIOACTIVE PIGMENTS FROM MARINE BACTERIA ISOLATED FROM COASTAL REGION OF KELWE-MAHIM, PALGHAR", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.7, Issue 11, page no. pp24-31, November-2020, Available at : http://www.jetir.org/papers/JETIREI06004.pdf