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Published in:

Volume 7 Issue 11
November-2020
eISSN: 2349-5162

Unique Identifier

JETIREI06004

Page Number

24-31

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Title

IDENTIFICATION AND CHARACTERIZATION OF BIOACTIVE PIGMENTS FROM MARINE BACTERIA ISOLATED FROM COASTAL REGION OF KELWE-MAHIM, PALGHAR

ISSN

2349-5162

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"IDENTIFICATION AND CHARACTERIZATION OF BIOACTIVE PIGMENTS FROM MARINE BACTERIA ISOLATED FROM COASTAL REGION OF KELWE-MAHIM, PALGHAR", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.7, Issue 11, page no.24-31, November-2020, Available :http://www.jetir.org/papers/JETIREI06004.pdf

Abstract

Marine bacteria though rich in pigments largely remain unexplored for its bioactive components. Synthetic pigments used in various industries pose various harmful effects, to overcome these problems it is important to explore varied natural sources for natural pigments. Pigmented bacterial isolates were screened for sediment sample of the coastal region of kelwe beach,palghar. 20 different bacterial isolates were obtained on st.zobells agar. From these 20 bacterial isolates, 2 visually pigmented bacteria orange and yellow pigment producing bacteria were further studied. The MIC of both microbial isolates were found to be at 8% NaCl and hence was moderate halotolerant in nature. Optimum growth of both pigment producing bacteria was found to be at pH 9. Pigment was extracted from bacteria using different solvents such as methanol, ethanol, acetone and chloroform, the maximum absorbance of the pigment was found to be 430nm and methanol was found to be the best solvent for maximum pigment extraction for both the isolates. The identification of pigment was done by chromatography technique and the Rf value was 0.48 and 0.58 for orange and yellow pigment, probably may contain zeaxanthin and lutein respectively. Antioxidant activity of pigment was checked by DPPH method, the percentage of scavenging activity of orange and yellow pigment was found to be 18.841% and 33.334% respectively. Antimicrobial activity of the extracted pigment was studied against E.coli, S.aureus and S.typhi by agar cup method and both pigment show maximum antimicrobial activity against only S.aureus

Key Words

Marine pigments, thin layer chromatography, Antioxidant, DPPH assay, Antimicrobial activity.

Cite This Article

"IDENTIFICATION AND CHARACTERIZATION OF BIOACTIVE PIGMENTS FROM MARINE BACTERIA ISOLATED FROM COASTAL REGION OF KELWE-MAHIM, PALGHAR", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.7, Issue 11, page no. pp24-31, November-2020, Available at : http://www.jetir.org/papers/JETIREI06004.pdf

Publication Details

Published Paper ID: JETIREI06004
Registration ID: 302488
Published In: Volume 7 | Issue 11 | Year November-2020
DOI (Digital Object Identifier):
Page No: 24-31
ISSN Number: 2349-5162

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Cite This Article

"IDENTIFICATION AND CHARACTERIZATION OF BIOACTIVE PIGMENTS FROM MARINE BACTERIA ISOLATED FROM COASTAL REGION OF KELWE-MAHIM, PALGHAR", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.7, Issue 11, page no. pp24-31, November-2020, Available at : http://www.jetir.org/papers/JETIREI06004.pdf




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