UGC Approved Journal no 63975

ISSN: 2349-5162 | ESTD Year : 2014
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Published in:

Volume 5 Issue 7
July-2018
eISSN: 2349-5162

UGC and ISSN approved 7.95 impact factor UGC Approved Journal no 63975

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Published Paper ID:
JETIR1807966


Registration ID:
186103

Page Number

448-453

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Title

IN VTIRO CALLUS PROLIFERATION STUDIES OF SPILANTHES ACMELLA L. ( MURR.) IN DIFFERENT PLANT GROWTH REGULATORS.

Abstract

Ayuerveda is historical gift given by India to the world. Where, it is huge compilation and work of medicinal practices and herbal drugs. Akarkarbha is also named as Akalkara which is Spilanthes acmella (Murr.) also mentioned in some texts of ayuervedic book. Its propagation by seed has so many consequences hence by using in vitro prorogation techniques this plant can be conserved. Micropropogation studies on various growth hormones reported callus formation for mass multiplication and in vitro pharmaceutical drug preparation. Experiment reported that among different explants leaf explant takes lower days (9.90 ± 0.40) )for callus formation with 80% callus response in MS medium fortified with BAP (2mg/l) than other combination of Auxins. The callus growth reported is massive healthy with 2300 mg fresh weight. Higher callus fresh weight (2600 mg) observed in BAP (3mg/l) in leaf explants. The callus is firm and friable texture with greenish and yellow with slight white in color. After callus formation this callus is supplemented with Auxin and Cytokinin for root and shoots formation, then after transfer it for hardening when good rooted plants are formed. Hardening response is seen higher 80% (Survival rate) in 2:1 proportion of cocopeat and soil in-vitro potting media.

Key Words

Spilanthes acmella, Callus, BAP, Kinetin. etc.

Cite This Article

"IN VTIRO CALLUS PROLIFERATION STUDIES OF SPILANTHES ACMELLA L. ( MURR.) IN DIFFERENT PLANT GROWTH REGULATORS.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.5, Issue 7, page no.448-453, July-2018, Available :http://www.jetir.org/papers/JETIR1807966.pdf

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2349-5162 | Impact Factor 7.95 Calculate by Google Scholar

An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 7.95 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator

Cite This Article

"IN VTIRO CALLUS PROLIFERATION STUDIES OF SPILANTHES ACMELLA L. ( MURR.) IN DIFFERENT PLANT GROWTH REGULATORS.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.5, Issue 7, page no. pp448-453, July-2018, Available at : http://www.jetir.org/papers/JETIR1807966.pdf

Publication Details

Published Paper ID: JETIR1807966
Registration ID: 186103
Published In: Volume 5 | Issue 7 | Year July-2018
DOI (Digital Object Identifier):
Page No: 448-453
Country: Dist Osmanabad, Maharashtra, India .
Area: Science & Technology
ISSN Number: 2349-5162


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