• MILAN A. NIMBALKAR
• K. Y. PATIL

Staphylococcus aureus is a causative agent of many types of infections throughout the world. This infections are of major concern because of the causative agent offering resistance to a wide range of commonly used antibiotics. Staphylococcus aureus which is gram +ve , Coagulase +ve, non spore forming bacteria is also responsible for the biofilm production. Biofilm constitute reservoir of pathogens and are associated with resistance to antimicrobial agents and chronic infections. In the present study 180 non duplicated clinical strains of S. aureus were screened by Tissue Culture Plate Method and Congo red Agar method. Antibiotic susceptibility of all these strains was carried out by Kirby bauer disc diffusion method. Tissue Culture Plate method 32% were highly biofilm producing and 46 % were moderately biofilm producing. Rest was non biofilm producing by tissue culture plate method. 38% were highly biofilm producing and 51% were moderately biofilm producing remaining 21% were non biofilm producing in case of congo red agar method.

antibacterial resistance, biofilm, congo red agar method. Staphylococcus aureus, tissue culture plate method.

"Detection of Biofilm development by Staphylococcus aureus and its role in antibiotic susceptibility pattern. ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.5, Issue 8, page no.375-383, August-2018, Available :http://www.jetir.org/papers/JETIR1808696.pdf

"Detection of Biofilm development by Staphylococcus aureus and its role in antibiotic susceptibility pattern. ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.5, Issue 8, page no. pp375-383, August-2018, Available at : http://www.jetir.org/papers/JETIR1808696.pdf