• Sushil Kumar
• Mahesh Babu
• Himshikha Yadav

During Dearth period it's necessary to own adequate supply of pollen substitutes to take care of bee colonies health. We compared three laboratory formulated diets (LFD): 1. Gram flour based, 2. Soya flour based diet, 3. Black pulse flour based diet, with bee collected pollen (as Natural feed) and Sugar syrup (used by beekeepers in most of parts of India during dearth period as bee feed) by measuring their effect on Haemolymph protein contents of young bees exclusively gulped up these diets, which may be a fast and cheap assay. The substitute diets included a non-soy-based, pollen substitute diet named LFD-1 having Gram flour (Cicer arietinum), a Soya based diet, named LFD-2 having Soya flour (Glycine max) and a Black pulse (Phaseolus mungo) based diet, named LFD-3 having pulse flour. All the diets got within the sort of patty to groups of 100 European honey bees Apis mellifera L. in Langstroth cages, maintained and fed from emergence until 10 days old. Sucrose, within the variety of syrup (50% ad libitum) was used as a protein free control diet. The results so obtained showed that LFD-1, LFD-2, LFD-3 and pollen increased protein content within the Haemolymph by factors of 2.50, 2.29, 1.69 and 1.76 respectively, over protein content analysed in bees fed only sucrose solution. The bees devoured LFD-1 and LFD-2 had their Haemolymph significantly enriched in protein as compared to group of bees fed on LFD-3 and the controls and to content slightly above those fed on pollen. All four proteinaceous diets were significantly superior to sugar syrup alone.

Pollen substitute, Laboratory Formulated diet (LFD), Honey bee, Protein, Haemolymph, Soybean, Cicer arietinum, Phaseolus mungo.

"Comparative Analysis of Protein content in Haemolymph of worker Honey Bees Apis Mellifera L. fed on laboratory formulated Diets ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.5, Issue 8, page no.997-1003, August-2018, Available :http://www.jetir.org/papers/JETIR1809A53.pdf

"Comparative Analysis of Protein content in Haemolymph of worker Honey Bees Apis Mellifera L. fed on laboratory formulated Diets ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.5, Issue 8, page no. pp997-1003, August-2018, Available at : http://www.jetir.org/papers/JETIR1809A53.pdf