UGC Approved Journal no 63975(19)

ISSN: 2349-5162 | ESTD Year : 2014
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Published in:

Volume 5 Issue 10
October-2018
eISSN: 2349-5162

UGC and ISSN approved 7.95 impact factor UGC Approved Journal no 63975

7.95 impact factor calculated by Google scholar

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Published Paper ID:
JETIR1810890


Registration ID:
190598

Page Number

701-715

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Title

Development of Rapid, sensitive, High Performance Liquid Chromatography-Tandem Mass spectrometry (HPLC-MS/MS) assay for the simultaneous determination of Bupivacaine and Procaine in Human Plasma

Abstract

A simple, rapid, sensitive, specific, LC-ESI-MS/MS assay for the simultaneous detection and accurate measurement of Bupivacaine (1-butyl-N-(2,6-dimethylphenyl)piperidine-2-carboxamide) and Procaine (4-aminobenzoic acid 2-diethylaminoethylester) in human plasma. The precipitation method was used to extract the analytes and IS from human plasma. The chromatographic resolution of Bupivacaine, Procaine and corresponding IS was achieved using gradient flow on C18 column. The total chromatographic run-time was 3 min. A linear response function was established for the range of concentration 1-1000ng/ml for Bupivacaine and Procaine. The intra-day and inter-day accuracy and precision values for Bupivacaine and Procaine met the acceptance as per regulatory guidelines.

Key Words

Bupivacaine, Gradient technique, LC-MS/MS, Procaine, Simultaneous estimation.

Cite This Article

"Development of Rapid, sensitive, High Performance Liquid Chromatography-Tandem Mass spectrometry (HPLC-MS/MS) assay for the simultaneous determination of Bupivacaine and Procaine in Human Plasma ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.5, Issue 10, page no.701-715, October-2018, Available :http://www.jetir.org/papers/JETIR1810890.pdf

ISSN


2349-5162 | Impact Factor 7.95 Calculate by Google Scholar

An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 7.95 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator

Cite This Article

"Development of Rapid, sensitive, High Performance Liquid Chromatography-Tandem Mass spectrometry (HPLC-MS/MS) assay for the simultaneous determination of Bupivacaine and Procaine in Human Plasma ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.5, Issue 10, page no. pp701-715, October-2018, Available at : http://www.jetir.org/papers/JETIR1810890.pdf

Publication Details

Published Paper ID: JETIR1810890
Registration ID: 190598
Published In: Volume 5 | Issue 10 | Year October-2018
DOI (Digital Object Identifier): http://doi.one/10.1729/Journal.18713
Page No: 701-715
Country: Tirupati, Andhra Pradesh, India .
Area: Chemistry
ISSN Number: 2349-5162
Publisher: IJ Publication


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