• Shridevi Devadiga
• Mohammed Mubeen Shaikh
• Vikas Gupta
• Ganesh Lad

Green nanotechnology is a multidisciplinary field that has emerged as a rapidly developing research area, serving as an important, reliable and eco-accommodating technique that emphasize on making the procedure for synthesizing nanoparticles which are hassle-free, non-hazardous, and especially environment friendly, in contrast with currently employed chemical and physical methods. This study reports the synthesis of silver and copper nanoparticles using leaf, flower, and seed extract of Calotropis gigantea as reducing, capping and stabilizing agent. A series of reactions was conducted using different types and concentration of plant extracts as well as varying temperature, pH and concentration of metal ions to optimize the reaction condition thus contributing to higher yield of the metal nanoparticles. Visual observation, UV-Visible Spectrophotometry and SEM were used to characterize the synthesized silver and copper nanoparticles. A strong surface plasmon resonance band was observed between 300-400 nm for silver and 300-350nm for copper. Images from the high-resolution Scanning Electron Microscopy (SEM) demonstrated spherical silver nanoparticles with an average size ranging from 65-70nm and copper nanoparticles with a size greater than 200 nm. The synergistic antimicrobial action of synthesized silver and copper nanoparticles was tested against Gram-positive and Gram-negative organisms.

Calotropis gigantea, SEM, variables, copper nanoparticles, silver nanoparticles, antimicrobial activity

"Biosynthesis, Characterization and Evaluation of Synergistic Antimicrobial Activity by Silver and Copper Nanoparticles Synthesized Using Plant Extract of Calotropis gigantea", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 3, page no.235-240, March-2019, Available :http://www.jetir.org/papers/JETIR1903G35.pdf

"Biosynthesis, Characterization and Evaluation of Synergistic Antimicrobial Activity by Silver and Copper Nanoparticles Synthesized Using Plant Extract of Calotropis gigantea", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 3, page no. pp235-240, March-2019, Available at : http://www.jetir.org/papers/JETIR1903G35.pdf