• Bindu C
• Rama Bhat P
• Mahesh Kumar K S

Abstract : This study has been undertaken to investigate the possibility of polyclonal antibody generation against Costus speciosus phytoconstituents. To test the anti plant extract antibody production in rabbits , an innovative immunogen preparation was made for improving immune response to plant extracts. The immunogen preparation method involves incorporating albumin binding synthetic peptide polymer to phytoconstituents to enhance the systemic life of antigen and improve immune response against the phytoconstituents . The antibody generation in rabbits was evaluated by quantitative SDS page and western blot as well as by quantitative indirect ELISA . This study indicated production of phytoconstituents specific antibodies which is an important tool for immunoassay development for detection of phytoconstituents in any invivo study sample matrix . This is the first report for generation of antibodies to phytoconstituents from Costus speciosus.

Costus speciosus, plant extract, polyclonal antibodies.

"Generation of polyclonal antibodies against Costus speciosus (Koen ex Retz.) Smith phytoconstituents using peptide conjugated immunogen", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.7, Issue 2, page no.1093-1096, February-2020, Available :http://www.jetir.org/papers/JETIR2002156.pdf

"Generation of polyclonal antibodies against Costus speciosus (Koen ex Retz.) Smith phytoconstituents using peptide conjugated immunogen", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.7, Issue 2, page no. pp1093-1096, February-2020, Available at : http://www.jetir.org/papers/JETIR2002156.pdf