Abstract
Punarnava is an important therapeutic drug in Ayurveda, Siddha, and Unani systems of medicine. In Ayurveda, Punarnava is regarded as a rejenuvating drug. It has been employed commonly since the ancient period traditionally by the native population for various ailments. This plant has a variety of applications such as laxative, antipyretic, stomachic, cardiotonic, expectorant, hypotensive, diuretic, antibacterial, anti-inflammatory, and kidney stimulant. Punarnava is potent medicine on asthma, jaundice, eye diseases, urine complaints and menstrual cramps. However, there is confusion regarding the real identity of ‘Punarnava.’ Rakta Punarnava is equated with B. diffusa L., Neel Punarnava with B. repanda, and Shvet Punarnava with B. erecta and Trianthema portulacastrum L. In the present work, detailed comparative morphology, anatomy, pharmacognosy, phytochemistry, and chromatographical studies, along with the biological activity of all four Punarnava species, have been carried out. The detailed studies of these species revealed that all these species are very distinct. The anatomical parameters, physico-chemical constants and chromatography studies carried out in the present investigation serves as measures of a quality control and fingerprinting for the four plants i.e. . B. diffusa, B. erecta B. repanda, and Trianthema portulacastrum L.