UGC Approved Journal no 63975(19)

ISSN: 2349-5162 | ESTD Year : 2014
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Published in:

Volume 5 Issue 9
September-2018
eISSN: 2349-5162

UGC and ISSN approved 7.95 impact factor UGC Approved Journal no 63975

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Published Paper ID:
JETIR2005119


Registration ID:
231577

Page Number

854-858

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Title

In vitro screening of proteinase inhibitors in methanol and water extract of Tridax procumbens

Abstract

Plant proteinase inhibitors accumulate in leaves and flowers in response to pathogen attack and various physiological stresses. These inhibitors have been exploited extremely in human health care system for preparation of drugs to control various disorders. In this study we studied the presence of proteinase inhibitors (trypsin, chymotrapsin, Helicoverpa armigera gut proteinase inhibitor) in leaves and flowers of Tridax procumbens. Water and methanol were used for isolation of protease inhibitors from leaves and flowers. The presence of inhibitor activity in aqueous and methanol extracts against trypsin, chymotrypsin and H. armigera gut proteinase was detected by using dot blot assay on X-ray film. Methanol extracts of leaves and flowers were found to be containing moderately prominent activity than aqueous extract. Both leaves and flowers extracts were observed more active against chymotrypsin as compared to trypsin and H. armigera gut proteinase. Both extracts were found to be unable of inhibition of all proteinase when incubation time extends from 15 to 30 min. Therefore it is necessary to characterise T. procumbens proteinase inhibitor for further applications in various field.

Key Words

Tridax procumbens, Proteinase inhibitors, Trypsin, Chymotrypsin, Dot-blot assay, H. armigera gut proteinase

Cite This Article

" In vitro screening of proteinase inhibitors in methanol and water extract of Tridax procumbens", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.5, Issue 9, page no.854-858, September 2018, Available :http://www.jetir.org/papers/JETIR2005119.pdf

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2349-5162 | Impact Factor 7.95 Calculate by Google Scholar

An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 7.95 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator

Cite This Article

" In vitro screening of proteinase inhibitors in methanol and water extract of Tridax procumbens", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.5, Issue 9, page no. pp854-858, September 2018, Available at : http://www.jetir.org/papers/JETIR2005119.pdf

Publication Details

Published Paper ID: JETIR2005119
Registration ID: 231577
Published In: Volume 5 | Issue 9 | Year September-2018
DOI (Digital Object Identifier):
Page No: 854-858
Country: ----, -, -- .
Area: Engineering
ISSN Number: 2349-5162
Publisher: IJ Publication


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