UGC Approved Journal no 63975(19)

ISSN: 2349-5162 | ESTD Year : 2014
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Published in:

Volume 7 Issue 10
October-2020
eISSN: 2349-5162

UGC and ISSN approved 7.95 impact factor UGC Approved Journal no 63975

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Published Paper ID:
JETIR2010400


Registration ID:
300529

Page Number

3054-3065

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Title

Partial purification of chromate reductase from an Alkaliphile Pseudomonas DL17 (JN595813.1)

Abstract

An attempt was made to purify the chromate reductase from an alkaliphile with 46 fold purification with 40 percent recovery. The molecular weight was found to be 20 kDa. The higher enzyme activity was observed at pH 7.5 to 8.5 and temperature 350C to 50 0C with K2Cr2O7 as a substrate. The thermal stability of chromate reductase was up to 700C. Metal ions have no significant inhibitory action on chromate reductase. No activity was observed at 80 0C during enzyme stability experiments. It was an interesting thing observed in this regard was the enzyme also shown nitro reductase activity. Km value is 0.065 and the Vmax is 0.3 indicating its high affinity towards potassium dichromate.

Key Words

Chromate reductase, Nitroreductase, Pseudomonas DL17, Purification, xenobiotics

Cite This Article

"Partial purification of chromate reductase from an Alkaliphile Pseudomonas DL17 (JN595813.1)", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.7, Issue 10, page no.3054-3065, October-2020, Available :http://www.jetir.org/papers/JETIR2010400.pdf

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2349-5162 | Impact Factor 7.95 Calculate by Google Scholar

An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 7.95 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator

Cite This Article

"Partial purification of chromate reductase from an Alkaliphile Pseudomonas DL17 (JN595813.1)", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.7, Issue 10, page no. pp3054-3065, October-2020, Available at : http://www.jetir.org/papers/JETIR2010400.pdf

Publication Details

Published Paper ID: JETIR2010400
Registration ID: 300529
Published In: Volume 7 | Issue 10 | Year October-2020
DOI (Digital Object Identifier):
Page No: 3054-3065
Country: Osmanabad, Maharashtra, British Indian Ocean Territory .
Area: Biological Science
ISSN Number: 2349-5162
Publisher: IJ Publication


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