• Aishwarya Avinash Shinde
• Sayli Jawale
• Vaishnavi zargad
• Sonali Tarde
• Deepak Musmade

Plant tissue culture techniques are the most frequently used Biotechnological tools for basic and applied purposes ranging from Investigation of plant developmental processes, functional gene studies, commercial Plant micropropagation , generation of transgenic plants with specific industrial and agronomical traits,plants breeding and crop improvement, virus elimination From infected material to render high quality healthy plant material, presevation and conservation of germplasm of vegetative propagated plant crops, and rescue of threatened or endangered plant species. The significant effect of some factors such as medium components, phytoharmoes , explant type, and light on the regeneration ability of explant , recent reports evidence the involvement of Molecular singnals in organogenesis and embryogenesis responses to explant wounding , induced plant cell death, and phytoharmoes interaction. The cultured cell and tissue can take several pathways. The pathways that leads to the production of true-to-type plants in large number are the preferred ones of commercial multiplication. The process of micropropagation is usually divided into several stages., propagation , initiation of explants , subculture of explants for proliferation, shooting and rooting, adhardening. This stage are universally applicable in large-scale multiplication of plants. The delivery of hardned small micropropagated plants to growsers and market also requires extra care.

Large scale propagations; metabolic engineering; plant cell culture; micropropagation; embryogenesis; mutliplication of cells; pathways.

"Plant Tissue culture ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.7, Issue 11, page no.229-239, November-2020, Available :http://www.jetir.org/papers/JETIR2011037.pdf

"Plant Tissue culture ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.7, Issue 11, page no. pp229-239, November-2020, Available at : http://www.jetir.org/papers/JETIR2011037.pdf