• Raajesh K. Yadav
• Lakha Ram
• Kumbha Ram Mahala
• Raaz K. Maheshwari

Andrographis paniculata is a promising new herb for the treatment of many diseases including HIV-AIDS and the myriad of symptoms associated with autoimmune disorders, and it has been used in bacterial dysentery, arresting diarrhea and in upper respiratory infections, tonsillitis, pharyngitis, laryngitis, pneumonia, tuberculosis, and pyelonephritis. Due to high medicinal property it’s having high market demand in Homeopathy as well as in Ayurveda. The natural propagation of these plants is very poor and their populations are declining with an alarming rate Furthermore, an increasing reliance on the use of medicinal plants in the industrialized societies has been traced to the extraction and development of several drugs and chemotherapeutics from these plants as well as from traditionally used rural herbal remedies. Systematic approaches were made in order to achieve multiple shoot induction from seedling segment viz. cotyleuonary node, epicotyledonary node of Andrographis paniculata. In Andrographis paniculata BAP 13.31µM/lit with IAA. (20 shoots with 33.5mm length) from seedling segments. Amongst all the concentration of BAP with IAA tried, 13.31µM/lit BAP+5.70µM/lit IAA was found the best for further multiplication of Andrographis paniculata shoots. Incorporation of PVP (100Mg/lit) ascorbic acid (50Mg/lit) and citric acid (100Mg/lit), checked browning of culture.

Andrographis paniculata; Autoimmune disorders; IAA; BAP; PVP

"Rapid in vitro multiplication, regereration and rooting of Kalnesg (Andrographis paniculata Nees.)", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.8, Issue 5, page no.g512-g527, May-2021, Available :http://www.jetir.org/papers/JETIR2105867.pdf

"Rapid in vitro multiplication, regereration and rooting of Kalnesg (Andrographis paniculata Nees.)", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.8, Issue 5, page no. ppg512-g527, May-2021, Available at : http://www.jetir.org/papers/JETIR2105867.pdf