• Manoj Kumar Mishra
• Kalpana Sharma

Acinetobacter spp.is oxidase negative, glucose, lactose, sucrose fermenting with an optimum growth pH of 6.0-7.0 and temperature of 370C. Some diarrheagenic Acinetobacter spp.strains have the ability to tolerate exposure to pH 2.0. Eosin Methylene Blue medium is suitable for isolation of Acinetobacter spp.from faeces and foods because of the ability to produce distinctive colonies having greenish metallic sheen and not produced by any other member of Enterobacteriaceae. The first β-lactamase was identified in Acinetobacter spp. prior to the release of penicillin for use in medical practice. The four major groups of β-lactams penicillin, cephalosporins, monobactams and carbapenems have a b-lactam ring which can be hydrolysed by β-lactamases resulting in microbiologically ineffective compounds. In Gram negative pathogens, b-lactamase production remains the most important contributing factor to β-lactam resistance. On the basis of mechanism of action, most common β-lactamases are divided into three major classes (A, C & D) depending on amino acid sequences. These enzymes act on many penicillins, cephalosporins and monobactams. Class B β-lactamases called as metallo beta lactamases (MBLs), act on penicillins, cephalosporins and carbapenems but not on monobactams.

Antibiotic resistance, β-lactamases Carbapenems, Motility, Physiochemical

"METALLO BETA LACTAMASES (MBL) PRODUCTION IN CLINICAL SAMPLE (URINE AND BLOOD) OF ACINETOBACTER SPECIES", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.8, Issue 6, page no.f512-f522, June-2021, Available :http://www.jetir.org/papers/JETIR2106773.pdf

"METALLO BETA LACTAMASES (MBL) PRODUCTION IN CLINICAL SAMPLE (URINE AND BLOOD) OF ACINETOBACTER SPECIES", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.8, Issue 6, page no. ppf512-f522, June-2021, Available at : http://www.jetir.org/papers/JETIR2106773.pdf