• Mandaloju Venkateshwarlu

In vitro axillary bud segments and shoot induction was achieved in one of the important medicinal plants. Shoot induction was monitored after 4-6 weeks of inoculation by counting the number of shoots induced from each explants. The advantage with micro propagation is most of the in vitro propagated plants of many important medicinal species were found to be uniform, showing less variation in the secondary metabolic content than their wild counter parts (Annaj and Andrzej S 2005) Shoot induction form axillary bud segments were surface sterilized and inoculated into MS media supplemented with various combinations of BAP L-Glutamil acid and KN. Tissue culture techniques are now becoming popular as alternative means of vegetative propagation. Micropropagation involves multiplication of genetically identical individual by asexual reproduction within a short span of time with tremendous potential for the production of high quality plant based medicines (Murch et al., 2000). The effect of benzyl amino purine in inducing shoot induction was already reported in some of the important medicinal plants (Komalavalli and Rao, 2000In Vitro propogation of Damaskrose Tabesh et al 2013 & Gago J 2014). The Axillary bud explants were inoculated on MS basal medium supplemented with various cytokinins i.e., BAP and NAA. Coconut water also had a role in triggering the formation of multiple shoots. Addition of BAP at 3.0 mg/l concentration or NAA at 3.0 mg/l to the MS basal medium, induced regeneration from the Tendril explants. Multiple shoot induction was achieved in one of the impartant medicinal plant of cucurbitacae family, Gmelina asatica (L). MS medium supplemented with 1.0 mg/l BAP + 2.0 mg/l NAA and 2.0 mg/l L-Glutamicacid was found to be optimum to induce shoots. The present study established reliable and reproducible protocol for rapid multiple shoot induction from axilary bud explants of Gmelina asatica (L) using different concentrations and combinations of cytokinins.

Multiple shoots, Axillary bud explants, BAP, L-Glutamic acid, NAA.

"CALLUS INDUCTION FROM AXILLARY BUD EXPLANTS OF Gmelina asatica (L) A Medicinal important plant.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.8, Issue 11, page no.c326-c329, November-2021, Available :http://www.jetir.org/papers/JETIR2111245.pdf

"CALLUS INDUCTION FROM AXILLARY BUD EXPLANTS OF Gmelina asatica (L) A Medicinal important plant.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.8, Issue 11, page no. ppc326-c329, November-2021, Available at : http://www.jetir.org/papers/JETIR2111245.pdf