• P. Venkateswara Rao
• Tanniru Rajesh

The present paper describes a simple, accurate and precise reversed phase High Performance Liquid Chromatography (HPLC) method for rapid and simultaneous quantification of Oritavancin. The chromatographic separation was achieved on Waters X-Bridge C18 (150x4.6mm, 3.5µ) column. Mobile phase contained a mixture of 0.1% Tri fluoro acetic acid and Acetonitrile in the ratio of 35:65 v/v, flow rate 1.0ml/min and UV detection at 370nm. The proposed method shows a good linearity in the concentration range of 50-300µg/ml of Oritavancin under optimized conditions. Precision and recovery study results are in between 98-102%. In the entire robustness conditions %RSD is below 2.0%. Degradation has minimum effect in stress condition and solutions are stable up to 24hrs. This method is validated for different parameters like Precision, linearity, accuracy, limit of detection (LOD), limit of quantification (LOQ), ruggedness, robustness and forced degradation study were determined according to the ICH Q2B guidelines. All the parameters of validation were found to be within the acceptance range of ICH guidelines.

RP-HPLC, Oritavancin

"DEVELOPMENT AND VALIDATION OF ORITAVANCIN IN PHARMACEUTICAL FORMULATION BY USING RP-HPLC TECHNIQUE", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.8, Issue 12, page no.e808-e813, December-2021, Available :http://www.jetir.org/papers/JETIR2112500.pdf

"DEVELOPMENT AND VALIDATION OF ORITAVANCIN IN PHARMACEUTICAL FORMULATION BY USING RP-HPLC TECHNIQUE", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.8, Issue 12, page no. ppe808-e813, December-2021, Available at : http://www.jetir.org/papers/JETIR2112500.pdf