• Sujata Gangawane
• Kalyani Dalvi
• Vaishali Salunke
• Dr. Gajanan Sanap
• Samruddhi Wagh

The study aimed to achieve enhanced targeted toxicity and cell-internalization of cisplatin-loaded deoxyribonucleic acid-nanothread (CPT-DNA-NT), mediate by scavenger receptors into Hel cells. DNA-NT was developed with stiff-topology utilizing circular-scaffold to encapsulate CPT. Atomic force analysis (AFM) characterization of the DNA-NT showed uniformity among the structure with a diameter of 50–150 nm and length of 300–600 nm. The winning fabrication of the DNA-NT was confirmed through native-polyacrylamide gel activity analysis, as large the molecular-weight (polymeric) DNA-NT did not split into constituting strands beneath applied current and voltage. The results of cell viability confirmed that blank DNA-NT had the tiniest quantity toxicity at the most effective concentration (512 nM) with a viability of ninety 2 as proof of its biocompatibility for drug delivery. MTT assay showed superior toxicity of CPT-DNA-NT than that of the free CPT because of the depot unleash of CPT once DNA-NT incorporation. The DNA-NT exhibited targeted cell internalizations with the controlled living thing unleash of CPT (from DNA-NT), as illustrated in confocal photos. Therefore, in vitro toxicity assessment through flow cytometry showed enhanced programmed necrobiosis (72.7%) with CPT-DNA-NT (compared to free CPT; sixty four.4%). CPT-DNA-NT, being poly-anionic, showed enhanced endocytosis via scavenger receptors

DNA, nanostructures, Self-assembly Nanocarriers Drug delivery, in vivo cancer treatments

"DNA based Nanocarriers for Cancer treatment", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.9, Issue 2, page no.e399-e405, February-2022, Available :http://www.jetir.org/papers/JETIR2202451.pdf

"DNA based Nanocarriers for Cancer treatment", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.9, Issue 2, page no. ppe399-e405, February-2022, Available at : http://www.jetir.org/papers/JETIR2202451.pdf