• Anmol Juyal
• Dr Uma Kumari

The genomic landscape shows marked variation in the distribution of a number of features, including genes, transposable elements, GC content, CpG islands and recombination rate. Mutations were found through polymerase chain reaction-single strand conformation polymorphism, and then changed DNA fragments were purified and multiplied in sequencing. BLAST and ORF finder were used to find out the amino changes in sequence. The study is about 1FDQ, a Fatty-Acid Binding Protein (FABP). Taken from protein database, it shows crystal structure of a Brain Fatty-Acid Binding Protein (B-FABP) which is related to neuronal differentiation during embryonal neurodevelopment the absence of which has been shown to affect learning, memory, emotion regulation in rats – showing symptoms similar to schizophrenia. Similar is another protein called, Neurofilament M, a major component in the pathogenesis of Parkinson's disease (PD) where mutation analysis of the NF-M gene has been done in 322 familial PD patients. Two polymorphisms (Ala475Thr and Gly697Arg) occurring at similar frequencies in PD patients in substitutions affect residues of the NF-M protein that are highly conserved among different species. In analysis rare variants of the NF-M gene may act as susceptibility factors for PD and functional analyses of the identified variations are warranted to decipher possible mechanisms in neurodegeneration. Python-based software, alongside many Python plugin tools, has been developed to enhance the utilities and facilitate drug design in PyMOL which was used to visualize the protein structure, along with RasMol- another commonly-used visualization software. Various molecular modeling modules for visualization and analysis enhancement, protein–ligand modeling, molecular simulations, and drug screening are present in PyMOL. The human brain has been utilising cellular and molecular biological techniques for generations, where the mechanisms underlying the development, differentiation and function of the human brain remain quite elusive.

IndexTerms- Gene expression, human neuron protein,structural analysis, data compilation,fatty-acid binding protein.

"Mutational And Structural Analysis Of Human Brain Representing Intraspecific Variability", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.9, Issue 3, page no.b225-b230, March-2022, Available :http://www.jetir.org/papers/JETIR2203130.pdf

"Mutational And Structural Analysis Of Human Brain Representing Intraspecific Variability", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.9, Issue 3, page no. ppb225-b230, March-2022, Available at : http://www.jetir.org/papers/JETIR2203130.pdf