• Dheeraj kumar
• Dr monika tyagi
• Sourobhi datta
• Richa Yadav

Carvacrol is considered to improve food safety and the health of customers, and have been registered by U.S. Food and Drug Administration and recognized as safe component. Carvacrol is the antimicrobial monoterpene phenolic compounds which contains with lipophilic and volatile secondary metabolites that can thus interact with the cell physicochemical properties. In this study, the antimicrobial efficacy of carvacrol oil-in-water emulsions of concentrations of 0.1, 0.5, 1.0, and 10% were examined on bacteriophage MS2; 1.0 and 10% carvacrol were also examined on Escherichia coli. In addition, carvacrol oil-in-water emulsion stabilized by a nonionic surfactant (Tween 80) and cationic surfactant (CTAB) or in combination with anionic surfactant (sodium dodecyl sulfate) in inactivation of MS2 was also investigated. In antiviral efficacy, carvacrol at 0.1, 0.5, 1.0 and 10% have no reduction on bacteriophage MS2 at 60min contact time. In antibacterial efficacy, the 1.0% concentration showed no bacteria colony reduction on E. coli while 10% carvacrol showed 7.2 log10 reduction at 60min contact time. At ionic surfactant on antiviral efficacy test, 0.1% sodium dodecyl sulfate (SDS) showed no reduction while 0.2% CTAB and 0.25% SDS showed slight antiviral reduction (0.6 and 0.55 log reduction) on the phage. Moreover, a 2.5 log10 reduction was observed at 0.5% SDS on MS2. Due to there is no antiviral reduction when we applied carvacrol along on MS2, thus we added an appropriate amount of cationic or anionic surfactant to investigate whether additional ionic surfactant can improve antiviral efficacy. In combination 1% carvacrol with surfactant study, incubated aliquot MS2 and cationic carvacrol emulsion contained (1% carvacrol plus 0.5% Tween 80 and 0.2% CTAB) at 60min, a 2 log10 reductions was observed on MS2. In anionic emulsion (contained 1% carvacrol plus 0.25 or 0.5% SDS), mixed separately 0.25 or 0.5% SDS with MS2 for 30 min and 60 min. At both 0.25 and 0.5% SDS, the ~4.6 log10 reduction were observed at two different contact time (30 and 60min). In this study, both cationic and anionic carvacrol emulsion can improve the antiviral efficacy, the results represent a step forward in improving food safety and reduce viral plaques colony by using carvacrol oil- in-water emulsion combining surfactant.

carvacrol emulsion organic compounds

"CARVACROL EMULSION ANTIMICROBIC EFFICACY ON BACTERIOPHAGE MS2 AND ESCHERICHIA ECOLI", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.9, Issue 7, page no.c103-c108, July-2022, Available :http://www.jetir.org/papers/JETIR2207213.pdf

"CARVACROL EMULSION ANTIMICROBIC EFFICACY ON BACTERIOPHAGE MS2 AND ESCHERICHIA ECOLI", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.9, Issue 7, page no. ppc103-c108, July-2022, Available at : http://www.jetir.org/papers/JETIR2207213.pdf