• Disha shinde
• Vinayak gaware
• Kaveri vaditake

A simple, rapid, and selective RP-HPLC method was developed for the estimation of vancomycin hydrochloride concentration in injectable dosage forms. The method was developed using Nucleosil C18 – (250 mm × 4.6 mm x 10-µm) with a mobile phase composed of phosphate buffer (pH 2.2), acetonitrile, and water in the ratio of 50:50 (by volume), respectively. The mobile phase was pumped isostatically HPLC system at a flow rate of 1 mL/min and quantification of the analyte was based on measuring its peak areas at 235 nm. The retention time for vancomycin hydrochloride was about 10.15. The reliability of the proposed HPLC procedure was validated to linearity, ranges, precision, accuracy, specificity, and detection limit. The calibration curve was linear in the range of 50% to 150% of the working concentration. The range for the analytical method was found to be 25 ppm to 75 ppm with a correlation coefficient of more than 0.9999. The proposed method proved to be selective and stability-indicating by the resolution of the analytes from the forced degradation (hydrolysis, oxidation, thermolysis, and photolysis) products. The validated HPLC method was successfully applied to the analysis of vancomycin hydrochloride in pharmaceutical dosage forms. The degradation products resulted from the storage of the drug under stress degradation conditions described by the International Conference on Harmonisation (ICH). Keywords: vancomycin hydrochloride, RP-HPLC, bulk dosage form, injectables

"Method development and validation of Vancomycin drug in bulk and dosage form by RP-HPLC technique", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.9, Issue 7, page no.c174-c189, July-2022, Available :http://www.jetir.org/papers/JETIR2207220.pdf

"Method development and validation of Vancomycin drug in bulk and dosage form by RP-HPLC technique", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.9, Issue 7, page no. ppc174-c189, July-2022, Available at : http://www.jetir.org/papers/JETIR2207220.pdf