• Godithala Pardha Saradhi
• Sudhakar Poda

The study examined the role of Vaccinium sect. Cyanococcus fruit extract as reducing and stabilizing agents in the biosynthesis of selenium nanoparticles (Bgn-SeNPs) and followed by focused on the assessment of preferential anticancer effects of Bgn-SeNPs. Vaccinium sect. Cyanococcus (VC) fruits were purchased from a nearby market in Vijayawada, Andhra Pradesh. The cold maceration technique was chosen oven Soxhlet extraction technique to obtain VC fruit extract. The biosynthesis of selenium nanoparticles (Bgn-SeNPs) was carried out using Vaccinium sect. Cyanococcus (VC) fruit extract as reducing and stabilizing agent. As-synthesized biogenic selenium nanoparticles (Bgn-SeNPs) were subjected to various physico-chemical characterization analyses by various nanotechnological approaches. UV-Visible spectroscopy absorbance peak for Bgn-SeNPs was noticed at 320 nm. XRD analysis revealed that Bgn-SeNPs was not exhibited crystalline nature and were found to have amorphous in nature. DLS pattern showed that Bgn-SeNPs were in the range of nano-sized, with an average size of 130 nm. Zeta potential analysis showed that Bgn-SeNPs were highly stable with a charge of -25 mV. SEM analysis showed that Bgn-SeNPs were irregular in shape, had a slight aggregation, and had a size range between 106 – 150 nm. Following, study focused on the assessment of preferential anticancer effects of Bgn-SeNPs and it was evaluated by related to toxicity towards normal cells by MTT, LDH, and micro-morphological assessments. Anticancer activity of Bgn-SeNPs was performed on various cancer cell line models, which include RAW 264.7 (mouse macrophage cells), A549 (human lung cancer cells), MDA-MB-231 (human breast cancer cells), and Caco-2 (human intestinal cells). The biocompatibility of Bgn-SeNPs was evaluated by the accomplishment of the cytotoxicity analysis on human normal kidney cells HEK-293. MTT assay showed that Bgn-SeNPs have dose-dependent effect on viability of cancer and normal cells. In MTT assay, IC50 values of Bgn-SeNPs (concentration required to inhibit 50% of cell viability) on RAW 264.7, A549, MDA-MB-231, and Caco-2 cancer cells were determined as 64.29 ± 3.71, 38.03 ± 2.14, 67.81 ± 3.05, and 54.73 ± 2.90 µg/mL, respectively. The Bgn-SeNPs have exhibited superior anticancer activity on A549 cells. In the LDH assay, superior LDH release was noticed in cancer cells at lower concentrations of Bgn-SeNPs. Whereas, Bgn-SeNPs induced the LDH release in normal at a higher dosage. The anticancer and cytotoxic effect of Bgn-SeNPs on cancer and normal cells were revealed by micro-morphological observation. The dose of Bgn-SeNPs required to induce detrimental changes in the micro-morphology of normal was quite higher related to cancer cells. The study concluded that as-synthesized Bgn-SeNPs were highly selective in inhibiting the cancer cells in relation to normal cells.

Selenium nanoparticles, Vaccinium sect. Cyanococcus, UV-Visible spectroscopy, Scanning electron microscope, Anticancer activity, MTT assay, LDH assay, Micro-morphological observation.

"PREFERENTIAL ANTICANCER ACTIVITY OF BIOGENIC SELENIUM NANOPARTICLES FROM VACCINIUM SECT. CYANOCOCCUS FRUIT EXTRACT", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.9, Issue 9, page no.b667-b676, September-2022, Available :http://www.jetir.org/papers/JETIR2209182.pdf

"PREFERENTIAL ANTICANCER ACTIVITY OF BIOGENIC SELENIUM NANOPARTICLES FROM VACCINIUM SECT. CYANOCOCCUS FRUIT EXTRACT", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.9, Issue 9, page no. ppb667-b676, September-2022, Available at : http://www.jetir.org/papers/JETIR2209182.pdf