• UMA KUMARI
• Shanu
• Devendra Kumar

Abstract: Identification and quantification of human insulin is increasingly requested for clinical monitoring, for anti-doping purposes. Indeed, insulin analogues may be abused for suicide or homicide in different way in forensic interest. The recent technological advances the development of new extraction techniques particularly for anti-doping analyses, detection of insulins in the first validated quantitative method for analysis human insulin and its six analogues (Lispro, Aspart, Glulisine, Glargine, Detemir and Degludec) in plasma. Sequence analysis using complete amino acid sequence of human insulin-like growth factor I (IGF-I), a polypeptide isolated from serum, has been determined.Analysis PDBsum the relative disposition of the α-helices (red cylinders) Insulin-like growth factor-1 (IGF-1) act as mediator of growth hormone (GH), that promoting cell growth and differentiation in childhood have an anabolic effect in adults. IGF-1 network of growth factors, receptors and binding proteins involved in mediating cellular proliferation, differentiation and Bioavailability of IGF-1 is affected by insulin-like growth factor binding proteins (IGFBPs) which bind IGF-1 in circulation. IGFs is a (also called C-domain) single polypeptide chains which hold the insulin folding. IGF-1 and IGF-2(except that they are expressed at different stages of physiological development) have similar functional.

Insulin, Structure Analysis, Validation, multi-domain model,COBALT, E-Value

"IDENTIFICATION AND QUANTIFICATION OF HUMAN INSULIN BY APPLYING CONSERVE DOMAIN SEARCH", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.10, Issue 4, page no.l129-l133, April-2023, Available :http://www.jetir.org/papers/JETIR2304B22.pdf

"IDENTIFICATION AND QUANTIFICATION OF HUMAN INSULIN BY APPLYING CONSERVE DOMAIN SEARCH", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.10, Issue 4, page no. ppl129-l133, April-2023, Available at : http://www.jetir.org/papers/JETIR2304B22.pdf