• Swapnil salve
• Dr.Lahu Hingne
• Dr.santosh kamble

A new simple, specific, precise and accurate reversed-phase liquid chromatography method, UV-Spectrophotometric and RP-High-Performance Liquid Chromatography method has been developed for the simultaneous estimation of Itraconazole. The separation was achieved on the C18 column using mobile phase consisting of mixture of (MEOH + 0.05 % OPA WATER) in ratio of 90:10. The detection was carried out at 268nm with flow rate of 0.9 ml/min. The retention time for Itraconazole was found 3.495 minutes respectively. The calibration curve was found linear (r2 = 0.9997) for RP- HPLC method. The UV- Spectrophotometric method was developed using Schimadzu 1800 UV– Visible spectrophotometer using methanol as a solvent. The method was found linear and the wavelength of detection for Itraconazole was 262 nm respectively. The methods were validated in accordance with International Conference On harmonization acceptance criteria for specificity, linearity, precision, accuracy, robustness and system Suitability. The excipients did not interfere in the determination of Itraconazole. The project study was focused towards stability indicating stress degradation study of itraconazole. Itraconazole was subjected for degradation under various stress conditions like acidic degradation, alkaline degradation, oxidative degradation. The percent degradation by alkaline hydrolysis was 47.78% and by acid hydrolysis was 44.88%. The oxidative degradation was 78.74%. Hence itraconazole was more prone to degradation by oxidative hydrolysis.

Degradation, Ultra-violet spectroscopy, HPLC, Mass spectroscopy

"Development and validation of stability indicating RP-HPLC and UV- Spectrophotometric method for estimation of Itraconazole in bulk and formulation", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.10, Issue 8, page no.a120-a125, August-2023, Available :http://www.jetir.org/papers/JETIR2308020.pdf

"Development and validation of stability indicating RP-HPLC and UV- Spectrophotometric method for estimation of Itraconazole in bulk and formulation", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.10, Issue 8, page no. ppa120-a125, August-2023, Available at : http://www.jetir.org/papers/JETIR2308020.pdf