• Tanvi Narkar
• Manish Bhat

L-asparaginase has proven to be an anti-cancer agent and several studies are being carried out to discover its novel sources. Oceans are vastly inhabited by various microorganisms producing secondary metabolites such as an enzyme. These enzymes are produced in extreme marine conditions such as alkaline pH, hyperthermal stability, barophilicity, and salt tolerance. Due to these factors marine L-asparaginase can find a wide range of applications in various industries. Several studies have been related to microbes producing L-asparaginase from Fish, sponges, and marine sediments. Marine crustaceans are comparatively an underexplored source. The main objective of this study is to isolate the L-asparaginase producing microorganism from a widely consumed species of crustaceans i.e., Metapenaeus dobsoni. Total 348 organisms were screened from which only eleven pigmented isolates were selected for the study. Isolates were screened by Rapid plate assay and enzyme assay, from which five isolates exhibited L-asparaginase activity. The selected isolate TN20 showed 99.47% similarity with Cornyebacterium callunae DSM 20147(T) by 16s rRNA gene sequencing. Phylogenetic analysis revealed that the isolate could be novel (Further analysis for accession number is under process). The optimized physical parameters were found as pH (7.0 ±0.3) at room temperature with shaking condition. Antioxidant activity of the enzyme produced from the isolate was studied by DPPH assay, it was observed as radical scavenging activity of 65.59%. The enzyme was partially purified by Ammonium sulfate precipitation and Dialysis method. A fermented food product Terasi, was prepared using Metapenaeus dobsoni its sensory analysis was carried out and found to be statistically significant. This is the first kind of report on L-asparaginase producing Cornyebacterium callunae from Marine crustaceans, Metapenaeus dobsoni. Further purification of the enzyme and its cytotoxicity studies will lead to novel findings.

L-asparaginase, Marine Crustaceans, Cornyebacterium callunae, Antioxidant property, Metapenaeus dobsoni, Terasi

"Isolation studies of L-Asparaginase producing microorganism from marine Crustacean, Metapenaeus dobsoni.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.10, Issue 8, page no.c585-c599, August-2023, Available :http://www.jetir.org/papers/JETIR2308267.pdf

"Isolation studies of L-Asparaginase producing microorganism from marine Crustacean, Metapenaeus dobsoni.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.10, Issue 8, page no. ppc585-c599, August-2023, Available at : http://www.jetir.org/papers/JETIR2308267.pdf