• Sonia Patharia

The current study aimed to find effect of infection with Aeromonas hydrophila and treated with extract of Curcuma longa on some biochemical parameter in Cirrhinus mrigal fish namely acid and alkaline phosphatise enzyme (ACP & ALP), alanine and aspartate amino transpharase enzyme (ALT & AST), total protein, glucose, and cholesterol. To accomplish this, C. mrigals infected with Aeromonas hydrophilla and treated with Cucuma longa for 20, 40, and 60 days. Aeromonas hydrophila were detected, isolated and identified from infected fish obtain from Anasagar Lake situated in Ajmer, Rajasthan (India). One year old mrigal carps obtained from the Aquaculture farm. Fishes acclimated for three days. For experiment 7 culture tanks (1 for control, 3 for infected, 3 for treated) containing 100 L aerated water were setup and divided in to 3 groups G1, G2, and G3. Further all groups divided in to 3 subgroups A, B, C (9 subgroups). 10 fishes in each tank for control, experimental and treated (20, 40, 60 days). Each fishes of G2 group injected intraperitoneally with 0.1ml concentration of 108 CFU/ml of Aeromonas hydrophila. Control fishes injected with 0.1ml of phosphate buffered saline. Fishes of G3 group injected with 0.1×108 CFU/ml Aeomonas hydrophila and treated with 5 mg/ml Curcuma longa. Regarding biochemical tests we noted significant decrease in the level of Total protein, Glucose, and cholesterol and significant increase in ACP [acid phosphatase] & ALP [alkaline phosphatase], ALT [alkaline transpharas] & AST [acid transpharase] in the infected group [G2] compared with a control group [G1] and significant increase in Total protein, Glucose, and cholesterol and significant decrease ACP [acid phosphatase] & ALP [alkaline phosphatase], ALT [alkaline transpharas] & AST [acid transpharase] in the treated group [G3] compared with infected group [G2]. The mean of groups of G1 & G2 and groups of G2 & G3 are significantly different at p<0.05. In this study infection with Aeromonas hydrophila can induce alteration in the level of some biochemical parameters and causes economic losses in production of fish.

Aeromonas hydrophila, ACP & ALP, ALT & AST, Cirrhinus mrigala, Cholesterol, Glucose, Total Protein.

"Effect on Biomarkers of Cirrhinus mrigala due to Aeromonas hydrophila along with remedial effects of extract of Curcuma longa ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.12, Issue 11, page no.a614-a625, November-2025, Available :http://www.jetir.org/papers/JETIR2511073.pdf

"Effect on Biomarkers of Cirrhinus mrigala due to Aeromonas hydrophila along with remedial effects of extract of Curcuma longa ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.12, Issue 11, page no. ppa614-a625, November-2025, Available at : http://www.jetir.org/papers/JETIR2511073.pdf