• Mathur Nadarajan Kathiravan

Characterization of xylanase produced by Aspergillus niger was done after extracting the xylanase from wheat bran (WB) which was used as a substrate for solid state fermentation (SSF) process. The extraction parameters for xylanase were optimized by central composite design (CCD). The maximum recovery of xylanase (1460 U/g) was obtained employing water (3.468 ml/g) as extractant at 200 rpm for 2h. Xylanase was found to be active in a vast range of pH from 3.6 to 9 with an optimum at 5. The optimum temperature of this xylanase was 50°C. In the presence of metal ions such as Ca2+ and Mn2+, the activity increased while Mg2+, Zn2+, Fe2+ and Co2+ showed a decreasing trend. Hg2+ ions completely inhibited the activity of xylanase. Surfactants caused a negative effect on the activity, when on tested with tween 80 the activity increased. The xylanase showed specific activity towards different xylans of which birchwood xylan yielding the maximum activity.

Aspergillus; Central composite design; Characterization; Extraction; Solid State Fermentation; Xylanase

"Acid Xylanase Production from Low Cost Solid State Fermentation Medium ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 3, page no.190-205, March-2019, Available :http://www.jetir.org/papers/JETIRAT06026.pdf

"Acid Xylanase Production from Low Cost Solid State Fermentation Medium ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 3, page no. pp190-205, March-2019, Available at : http://www.jetir.org/papers/JETIRAT06026.pdf