• Priyanka Haribhau Gogawale
• Jyoti Vijay Yande
• Sakharam Damu Aghav

Chlorophyll is a molecule produced by plant, algae and cyanobacteria and helps in conversion of light energy into chemical energy. Leaf chlorophyll content furnishes vital information and physiological status of plant. The green leaves changes color due to chlorophyll biodegradation. The chlorophyll a and chlorophyll b biodegradation pathways consist of large number of steps. The objective of present study is to compare degradation of chlorophyll content for three fresh leaves. Fresh leaves of spinach, coriander and lemongrass tea were used for the extraction of chlorophyll. The chlorophyll was extracted in 80% acetone and 20% distilled water. Filtered solution was used to detect chlorophyll-a, chlorophyll-b and total chlorophyll from visible spectrophotometer LI 722. Absorbance of chlorophyll contents was measured daily for twelve days continuously. Similarly filtered solution was kept in beaker and He-Ne laser light (5 mW,632.8 nm) was irradiated on solution for 1 min to 10 min with 1 min of interval. The arrangement was done in dark room to avoid stray light contamination with chlorophyll. For every min absorption study was carried out and chlorophyll a, chlorophyll b and total chlorophyll content was estimated. It had been observed that degradation of chlorophyll b is more than that of chlorophyll a .in all cases. Day wise degradation study was carried for spinach and lemon grass tea leaves, temperature wise degradation was studied for coriander leaves and laser effect was studied for spinach, lemon grass tea and coriander leaves. In all measurements (day wise degradation, temperature wise degradation, irradiation using He-Ne laser degradation), it was observed that total chlorophyll content decreases with days, temperature and time of exposure respectively.

Chlorophyll, Degradation, Spinach, Lemon grass, coriander, laser.

"COMPARATIVE STUDY OF CHLOROPHYLL DEGRADATION FOR SPINACH, CORIANDER AND LEMONGRASS TEA LEAVES", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 5, page no.56-60, May - 2019, Available :http://www.jetir.org/papers/JETIRCP06009.pdf

"COMPARATIVE STUDY OF CHLOROPHYLL DEGRADATION FOR SPINACH, CORIANDER AND LEMONGRASS TEA LEAVES", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 5, page no. pp56-60, May - 2019, Available at : http://www.jetir.org/papers/JETIRCP06009.pdf