• Vikas Nanekar
• Varsha Shriram
• Vinaykumar

Eulophia nuda Lindl. (Orchidaceae), a medicinally important perennial orchid with underground tubers used against tumors, scrofulous glands of the neck, bronchitis, blood diseases, rheumatoid arthritis besides as blood purifier, appetizer, vermifuge by folklores. Antioxidant, DNA damage protecting activity and antiproliferative activity of this plant had been reported earlier. Present work deals with screening of secondary metabolite of Eulophia nuda Lindl., HPTLC profiling, and detection of antioxidant compounds using TLC. Results revealed most of secondary metabolites including phenols flavonoids, tannins and alkaloids etc were present in ethyl acetate extract followed by methanol. The DPPH reagent was used for the detection of active antioxidant molecules on thin layer chromatograph, developing an easy and useful technique for isolation of active molecules. Clear zone (white spots) against violet background of DPPH indicated and confirmed the presence of antioxidant molecules. Ethyl acetate extract was showing highest DPPH radical scavenging activity and clear white spot on TLC plate. Thus ethyl acetate extract can be used further for the isolation of antioxidant molecules.

medicinal orchid, HPTLC profiling, secondary metabolites, DPPH

"SCREENING OF SECONDARY METABOLITES OF EULOPHIA NUDA LINDL., HPTLC PROFILING, AND DETECTION OF ANTIOXIDANT COMPOUNDS USING TLC.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.6, Issue 5, page no.279-282, May - 2019, Available :http://www.jetir.org/papers/JETIRCP06050.pdf

"SCREENING OF SECONDARY METABOLITES OF EULOPHIA NUDA LINDL., HPTLC PROFILING, AND DETECTION OF ANTIOXIDANT COMPOUNDS USING TLC.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.6, Issue 5, page no. pp279-282, May - 2019, Available at : http://www.jetir.org/papers/JETIRCP06050.pdf