• PAWAR S. B
• CHAVAN A.M

Onion is one of important commercial vegetable crops grown in worldwide. India is second most onionproducer. In India Maharashtra is leading state. Present study was investigated of the Analysis of genetic variability of Fusarium sps, from pathogenic fungi by using (ITS) as molecular markers. Fusarium species are among the most commonly occurring and economically important member of micro-fungi. The aim of this study was to estimate the genetic diversity. collected from various places from Maharashtra by usingon colony appearance of shape and spore morphological characters and The obtained DNA was qualitatively and quantitatively analyzed using Nano Drop ND-1000 Spectrophotometer, the molecular marker for characterizing the natural variation amongst Fusarium spp. isolatesusing (ITS -1 & ITS-4) as DNA barcoding of Fusarium sp. The Phylogenetic tree using nearest neighbor joining method, Sequences of Fusarium sps were grouped into three clusters. The largest cluster shows genetic similarity amongs ten species of Fusarium,

Fusarium, Onion, genetic variability, ITS molecular markers,

"ANALYSIS OF GENETIC VARIABILITY OF FUSARIUM SPECIES ON ONION USING ITS MARKERS", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.8, Issue 12, page no.417-423, December-2021, Available :http://www.jetir.org/papers/JETIRFI06071.pdf

"ANALYSIS OF GENETIC VARIABILITY OF FUSARIUM SPECIES ON ONION USING ITS MARKERS", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.8, Issue 12, page no. pp417-423, December-2021, Available at : http://www.jetir.org/papers/JETIRFI06071.pdf