• C. Elizabeth Rani
• Jeevitha
• K.K.Kavitha
• Senthil Kumar

Oil pollution is an environmental problem of increasing importance. Hydrocarbon-degrading microorganisms, adapted to grow and thrive in oil-containing environments, have an important role in the biological treatment of this pollution. Bioremediation is an efficient tool practiced now a day for the removal of contaminants from the contaminated soil and water. In the present study, an attempt has been made to investigate the bioremediation of petroleum hydrocarbons using bio surfactants produced by Streptomycetes. Microbes such as bacteria and fungi were isolated from the effluent and oil contaminated soil. They were identified by phenotypic methods and we found out two potential bacteria namely Pseudomonas fluorescence, Pseudomonas sps, fungi Aspergillusfumigatus, and a StreptomyceteRothiamuscilagenosa. Nature of biosurfactant produced by all the four chosen organisms were characterized and it was found to be glycolipopetides and Lipopetidebiosurfactant. Efficiency of bioremediation process was superior by the biosurfactant produced from Rothiamuscilagenosa, isolated from oil contaminated soil. Alkane monooxygenase (alkB) gene expression study was carried out and the gene was expressed in the selected Streptomycete, Rothiamuscilagenosa. Based on the present study it can be concluded that the biosurfactant produced from Rothiamuscilagenosa has the potential to remediate oil pollution.

Bioremediation, biosurfactants, Petroleum Hydrocarbons, soil, Alkane monooxygenase

"Bio Prospecting of Multipotent Bacterial Biosurfactant for the Remediation of Hydrocarbons", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.5, Issue 12, page no.85-88, December-2018, Available :http://www.jetir.org/papers/JETIRQ006018.pdf

"Bio Prospecting of Multipotent Bacterial Biosurfactant for the Remediation of Hydrocarbons", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.5, Issue 12, page no. pp85-88, December-2018, Available at : http://www.jetir.org/papers/JETIRQ006018.pdf