• Motireddy Srinivasulu Reddy
• Yenukolu Aparna
• Shaik Haleema Banafsha

Augmenting Biofloc systems with the addition of Probiotics have been proven to improve not only the water quality and also enhance shrimp performance interms of growth rates and productivity. In the present study, we aimed to evaluate the effects of addition of both Probiotics and Bioflocs on the Antioxidants status and Haemocyte population in L. vannamei. All the antioxidant enzymes and haemolymph parameters were found to be significantly (p<0.05) enhanced/altered in the Hepatopancreas, Gill and Haemolymph tissues of L. vannamei in the Probiotics blended Biofloc treatments than the Control. These results indicate that microbial protein from recycled waste functions as nutrient, growth promoter and antioxidant stimulator in shrimp reared in BFT systems. Three Experimental diets (ED-1) with combined use of both Probiotics (Bacillus licheniformis & Lactobacillus rhamnosus @ 10 billion cfu/kg feed) with Molasses, Tapioca and Maize flour were added as Carbon sources for the production of Bioflocs. ED-2 with Probiotics and Molasses, Maize flour and Sucrose combinations. ED-3 with Probiotics and Molasses, Tapioca, Maize flour and Sucrose combinations and conducted 120 day feeding trail experiments in field. The data from the Probiotic blended Biofloc fed prawn Experimental trails clearly show that both Probiotics and Bioflocs were able to effectively nullify the ROS production mechanisms by inducing appropriate antioxidant enzyme activities at the tissue and cellular level. This in turn promoted the restoration of normal physiological and biochemical processes, which in turn led to the efficient uptake, utilization, and subsequent absorption of feed, which in turn facilitated the enhancement of growth rate. Both Probiotic and Biofloc distinctly cause the pathogenic forms to be effectively removed from the shrimp’s tissues and haemolymph, returning the physiological state to normal allowing for maximum growth. Theerfore, using Probiotics and Bioflocs separately or together will encourage the modification of the antioxidant system to promote favourable production and higher yields.

Antioxidant enzymes, Total Haemocyte Count, Hepatopancreas, Gill, Haemolymph

"ANTIOXIDANT STATUS OF PACIFIC WHITE SHRIMP LITOPENAEUS VANNAMEI : MODULATION DURING FEEDING WITH PROBIOTIC BLENDED BIOFLOCS", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.11, Issue 5, page no.c137-c164, May-2024, Available :http://www.jetir.org/papers/JETIR2405217.pdf

"ANTIOXIDANT STATUS OF PACIFIC WHITE SHRIMP LITOPENAEUS VANNAMEI : MODULATION DURING FEEDING WITH PROBIOTIC BLENDED BIOFLOCS", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.11, Issue 5, page no. ppc137-c164, May-2024, Available at : http://www.jetir.org/papers/JETIR2405217.pdf