• Dr. Anitha Thomas
• Nagasri Ponnala

Abstract: This study explores the antimicrobial potential of Camellia sinensis and Azadirachta indica leaf extracts against bacteria that form oral biofilms. By examining their efficacy in combating oral biofilm formation, the research aims to provide valuable insights into alternative oral health management approaches. Both Camellia sinensis and Azadirachta indica have been traditionally known for their antimicrobial properties, and this study seeks to validate their effectiveness in addressing oral biofilm-related issues. The findings could pave the way for the development of natural antimicrobial strategies in oral care. To scientifically evaluate the efficacy of these plants against oral biofilm-forming bacteria, the study conducted phytochemical screening and assessed the antimicrobial activity of methanolic leaf extracts. Various bacterial species isolated from patients with dental caries and a standard strain Streptococcus salivarius (MTCC13009) were targeted for evaluation. Phytochemical screening revealed the presence of several bioactive compounds in both extracts, with catechin from Camellia sinensis and nimbidin from Azadirachta indica identified. Biofilm production was assessed through qualitative and quantitative methods, and the antimicrobial activity of the extracts was tested using agar-well diffusion and disc diffusion methods, comparing them with a standard antibiotic Chloramphenicol. Camellia sinensis demonstrated greater antimicrobial activity compared to Azadirachta indica. Additionally, a mouthwash was formulated using Camellia sinensis extract and compared with commercial Chlorhexidine mouthwash at different concentrations. Statistical analysis using ANOVA was performed, indicating significant antimicrobial activity for the Camellia sinensis mouthwash.

Oral biofilm formation, antimicrobial potential, Camellia sinensis, Azadirachta indica, phytochemical screening.

"EVALUATIVE ANALYSIS OF ANTIBACTERIAL EFFICACY OF CAMELLIA SINENSIS AND AZADIRACHTA INDICA ON ORAL BIOFILM-FORMING BACTERIA", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.11, Issue 5, page no.c346-c362, May-2024, Available :http://www.jetir.org/papers/JETIR2405242.pdf

"EVALUATIVE ANALYSIS OF ANTIBACTERIAL EFFICACY OF CAMELLIA SINENSIS AND AZADIRACHTA INDICA ON ORAL BIOFILM-FORMING BACTERIA", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.11, Issue 5, page no. ppc346-c362, May-2024, Available at : http://www.jetir.org/papers/JETIR2405242.pdf