• Monalisha Paul
• Satarupa Bhattacherjee
• Dr. Suman Kumar Halder
• Dr. Sandip Kumar Sinha

Smoking and smokeless tobacco (Nicotine) intake major risk factor for gastrointestinal disorders, mucosal damage, changes in gut irrigation, and impaired mucosal immune response, inflammatory bowel diseases, and several clinical abnormalities in digestive system. Soluble dietary fibre (SDF) capable of improving the gut health by increasing anaerobic bacterial growth. Zinc oxide nanoparticles and fructooligosaccharides (FOS) are well-known antioxidants and induces the good gut bacterial growth. Present study was designed to investigate the capability of nano-composite in between Zinc oxide nanoparticles-FOS (ZnOFNC) to protect intestine from nicotine induced damages. Estimated parameters were oxidative stress measurement and histological study of large and small intestine tissues and quantitative measurement of plasma SCFA level of selective groups (Gr-I: Control, Gr.-II Nicotine treated, Gr.-III Nicotine plus FOS and Gr.-III Nicotine plus ZnOFNC). The result of this study expressed that plasma SCFA level of Gr-II were decreased and increased the ROS generation compared to Gr-I (significant level was P<0.05 in all cases). Whereas the Gr.-III, ZnOFNC showed its ability to expand the antioxidant level and diminished the ROS level and increased the plasma SCFA level significantly (P<0.05). Nicotine-induced (Gr.-II) histological changes were also recovered in Gr.-III similar to control. Throughout the several experiments like biochemical, histological and plasma SCFA level measurement it is indicated that ZnOFNC may have some potential power to diminish nicotine-induced alterations in gut health.

Bacterial growth, Nanoconjugate, Fructo-oligosaccharides, Oxidative stress, Nicotine, Short chain fatty acids, Zinc oxide, Intestinal tissue

"CURATIVE EFFECTS OF ZINC OXIDE NANOPARTICLES-FOS COMPOSITE ON NICOTINE TEMPTED INTESTINAL OXIDATIVE STRESS WITH HISTOLOGICAL FEATURES AND SERUM SHORT CHAIN FATTY ACIDS MEASUREMENT.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.10, Issue 10, page no.f532-f543, October-2023, Available :http://www.jetir.org/papers/JETIR2310465.pdf

"CURATIVE EFFECTS OF ZINC OXIDE NANOPARTICLES-FOS COMPOSITE ON NICOTINE TEMPTED INTESTINAL OXIDATIVE STRESS WITH HISTOLOGICAL FEATURES AND SERUM SHORT CHAIN FATTY ACIDS MEASUREMENT.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.10, Issue 10, page no. ppf532-f543, October-2023, Available at : http://www.jetir.org/papers/JETIR2310465.pdf