• Md. Nizamuddin Ansari
• Chandan Kr. Singh
• Nidhi Kumari
• Md. Naseem

Diabetes is a global problem, the treatment of which is recommended by various homeopathic and herbal drugs. There are large number of plants which are recommended for its treatment in herbal system of medicine, Vernonia divergensBenth (Fam. – Asteraceae)., commonly known as insulin plant is a potent sugar killer and is used as an excellent medicine for diabetes mellitus. This plant has a restricted distribution in Muzaffarpur and some diabetic people grow this plant in their courtyards. Keeping in view its officinal use, the in vitro studies of this plant were being under taken to develop a protocol for mass propagation as well as to analyse its biochemical constituents. Regeneration of shoots and callus differentiation was obtained using shoot-tip and stem segment of Vernonia divergens as explants. Sterilized explants were cultured on MS (Murashige & Skoog, 1962) medium containing 0.8% agar, 3% sucrose and different combinations and concentrations of auxin and cytokinin. Technique have been developed for shoot regeneration directly from stem & shoot-tip explants as well as shoot regeneration from callus. 2 mgl-1 each of 2,4-D & Kn was most effective and induced the formation of direct shoots in culture of shoot-tip. 2,4-D (1-5 mgl-1) alone or 2,4-D + Kn resulted in callus differentiation from both the explants. Highest % of result was obtained on 2 mgl-1 each of 2,4-D & Kn. Callus was creamy white, hydrated and crystalline in appearance. Callus turned brown on higher concentration (5 mgl-1) of 2,4-D on subculture. Rooting of shoots was obtained on rooting medium (RM, ½ MS salt + full strength vitamins & amino acids) supplemented with 1mgl-1 NAA & 2mgl-1 IBA technique was more promising in woody shoots and plantlets were successfully transfer to soil and survive well in nature. Work are in progress to develop protocol for isolation of active constituents of pharmaceutical importance known for antidiabetic property.

Callus, Herbal, mellitus, protocol, restricted, technique, Vernonia.

"In vitro Plant Regeneration from nodal and Shoot-tip explants of Vernonia divergens ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.5, Issue 5, page no.1100-1107, May-2018, Available :http://www.jetir.org/papers/JETIR1805924.pdf

"In vitro Plant Regeneration from nodal and Shoot-tip explants of Vernonia divergens ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.5, Issue 5, page no. pp1100-1107, May-2018, Available at : http://www.jetir.org/papers/JETIR1805924.pdf