• Deepak K. Rahi
• Ekta Chaudhary
• Deepika Malik

Exopolysaccharides are high molecular weight compounds secreted by the organisms into their surroundings. These exopolysaccharides have found a wide applicability as food stabilizers, gelling agents, emulsifiers, flocculants, anti-oxidants, immune-modulators etc. The EPSs secreted by the organisms vary in their monosaccharide composition, solubility, surface structures, thermal stability, molecular weight, and presence or absence of certain functional groups. The present study deals with the physical and chemical characterization of the EPS produced by a root nodule isolate Enterobacter cloacae. The thin layer chromatography (TLC) revealed that the test EPS is composed of glucose and mannose residues. The differential scanning colorimetry (DSC) analysis revealed that the EPS was stable upto a temperature of 106ºC. Scanning electron microscopy (SEM) was done to determine the surface structure which revealed that the test EPS has a rough granular surface. It was also found that the EPS was soluble only in water and insoluble in organic solvents. Among the molecular parameters, Fourier Transfrom Infra-red (FT-IR) revealed the presence of carboxylic stretch, C-OH bend, CH3 bend, alcohol stretch, alkynyl stretch, and aromatic stretch in the test EPS. The X-ray diffraction (XRD) profile revealed that the test EPS is partially amorphous and partially crystalline in nature. Two distinct peaks with different molecular weight were observed in the MALDI-TOF MS analysis.

Enterobacter cloacae, EPS, Phaseolus vulgaris, TLC, DSC, FTIR

"Characterization of Exopolysaccharide (EPS) produced by Root Nodule isolate Enterobacter cloacae, isolated from Phaseolus vulgaris", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.5, Issue 9, page no.462-467, September-2018, Available :http://www.jetir.org/papers/JETIR1809715.pdf

"Characterization of Exopolysaccharide (EPS) produced by Root Nodule isolate Enterobacter cloacae, isolated from Phaseolus vulgaris", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.5, Issue 9, page no. pp462-467, September-2018, Available at : http://www.jetir.org/papers/JETIR1809715.pdf