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Published in:

Volume 5 Issue 12
December-2018
eISSN: 2349-5162

Unique Identifier

JETIR1812982

Page Number

581-589

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Title

Antibacterial and Phytochemical Studies with Cytotoxicity assay of Kalanchoe pinnata leave extract against Multi-drug Resistant Human Pathogens Isolated from UTI

ISSN

2349-5162

Cite This Article

"Antibacterial and Phytochemical Studies with Cytotoxicity assay of Kalanchoe pinnata leave extract against Multi-drug Resistant Human Pathogens Isolated from UTI", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.5, Issue 12, page no.581-589, December-2018, Available :http://www.jetir.org/papers/JETIR1812982.pdf

Abstract

UTI is one of the most common infections and more common in women than in men. Overuse of antibiotics has developed numerous defense mechanisms against chemical antimicrobial agents by bacterial pathogens; hence resistance to old and newly produced drugs is on the rise. The phenomenon of antibiotic resistance exhibited by the pathogenic microorganisms has led to the need for screening of several medicinal plants for their potential antimicrobial activity. Thus the present study was undertaken to investigate the antibacterial activity of different solvent (Methanol, Ethanol, Acetone, Petroleum ether and Water) extract of Kalanchoe pinnata leaves against multi-drug resistant UTI pathogens isolated from urine samples collected from patients suffering from UTI. Antimicrobial susceptibility test of 515 urinary isolates against 12 different antibiotics was performed to select multi drug resistant bacterial species and 7 different highly resistant bacterial species were selected for this study. In-vitro antibacterial activity was screened by well diffusion method against selected MDR bacterial species. All the selected extracts showed good antibacterial activity against all the selected MDR UTI pathogens in which water extract has greater antibacterial activity against all tested uropathogens. Preliminary phytochemical analysis showed presence of tannin, flavonoids, carbohydrates, protein, terpenoids and cardiac glycosides. Results of Cytotoxicity test performed by MTT assay and Viability assay showed less toxicity and more than 80% viability. Results of the present study showed that K. pinnata can be used as a potential source for drug development for the treatment of Urinary Tract Infection caused by multi drug resistant bacteria.

Key Words

UTI, Antibacterial activity, Phytochemical analysis, Kalanchoe pinnata, Cytotoxicity assay

Cite This Article

"Antibacterial and Phytochemical Studies with Cytotoxicity assay of Kalanchoe pinnata leave extract against Multi-drug Resistant Human Pathogens Isolated from UTI", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.5, Issue 12, page no. pp581-589, December-2018, Available at : http://www.jetir.org/papers/JETIR1812982.pdf

Publication Details

Published Paper ID: JETIR1812982
Registration ID: 193971
Published In: Volume 5 | Issue 12 | Year December-2018
DOI (Digital Object Identifier):
Page No: 581-589
ISSN Number: 2349-5162

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Cite This Article

"Antibacterial and Phytochemical Studies with Cytotoxicity assay of Kalanchoe pinnata leave extract against Multi-drug Resistant Human Pathogens Isolated from UTI", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.5, Issue 12, page no. pp581-589, December-2018, Available at : http://www.jetir.org/papers/JETIR1812982.pdf




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